湖北医药学院学报2025，Vol.44Issue(6)：687-691,封2,6.DOI:10.13819/j.issn.2096-708X.2025.06.006
羊水间充质干细胞治疗宫腔粘连的实验研究
Therapeutic Effect of Amniotic Fluid-Derived Mesenchymal Stem Cells on Intrauterine Adhesions
摘要
Abstract
Objective To investigate the therapeutic effect of amniotic fluid-derived mesenchymal stem cells(AF-MSCs)in a mouse model of intrauterine adhesions(IUA),and to reveal their potential mechanism by combining bioinformatics.Methods An IUA model was established using mechanical scraping followed by treatment with 95%ethanol.Two weeks af-ter surgery,AF-MSCs were injected into the uterine cavity,and uterine tissues were collected after another two weeks.En-dometrial thickness was assessed via HE staining,and we compared the fibrotic area using Masson staining.The expressions of CK19 and Vimentin were detected by immunohistochemistry,while differentially expressed RNAs were identified through RNA sequencing.Results Treatment with AF-MSCs resulted in a reduction in the extent of IUA,an increase in endometri-al thickness,and a decrease in the degree of fibrosis.The mechanism may be related to the up-regulation of the expression of genes related to ovarian sex hormone secretion and cell proliferation.Conclusion AF-MSCs significantly promote the proliferation of endometrial cells and improve the fibrosis associated with IUA,which provides a theoretical basis for AF-MSC therapy in the treatment of IUA.关键词
羊水间充质干细胞/宫腔粘连/子宫内膜细胞增殖Key words
Amniotic fluid-derived mesenchymal stem cells/Intrauterine adhesions/Proliferation of endometrial cells引用本文复制引用
DENG Chun-Lei,TAN Kai-qi,HUANG Zhi-Yi,LUO Mei,LING Sheng-tao..羊水间充质干细胞治疗宫腔粘连的实验研究[J].湖北医药学院学报,2025,44(6):687-691,封2,6.基金项目
湖北省自然科学基金创新发展联合基金项目(2025AFD236) （2025AFD236）
湖北医药学院研究生科技创新项目(YC2024054) （YC2024054）
十堰市科技局引导性项目(24Y037) （24Y037）
