湖北医药学院学报2025，Vol.44Issue(6)：692-695,702,封4,6.DOI:10.13819/j.issn.2096-708X.2025.06.007
肝组织氨基酸含量变化推断死亡时间的初步研究
Inferring Postmortem Interval Based on Changes in Amino Acids in Liver Tissue:A Preliminary Study
摘要
Abstract
Objective To detect the temporal variations in amino acids in postmortem liver tissue using gas chromatography-mass spectrometry(GC-MS)and to investigate the optimal equation for inferring the postmortem interval(PMI).Meth-ods A model simulating postmortem changes in isolated pig liver tissue was established,maintaining a constant temperature of 25℃ and humidity of 75%.Morphological assessments,including measurements of the total interstitial tissue area and total collagen fiber content,were conducted at 12-h intervals spanning from 0 h to 120 h PMI.Concurrently,samples were collected for amino acid extraction,and GC-MS was employed to analyze the correlation between changes in amino acids and PMI,ultimately establishing an optimal inference equation.Results The total interstitial tissue area,total collagen fi-ber content,and amino acid levels in isolated liver tissue exhibited temporal changes in relation to PMI.Notably,GC-MS revealed a strong correlation between alanine,leucine,valine,and PMI,yielding the inference equation:YPMI=11·456 Xleucine+13.605Xalanine+14.162Xglycine+6.110(R2=0.922,SE=10.629).Conclusion This study preliminarily validates the potential of using amino acids in liver tissue as a novel approach for inferring PMI during the early to middle stages of de-composition,suggesting promising application prospects.关键词
法医病理学/死亡时间/气质联用/尸体化学Key words
Forensic pathology/Postmortem interval/GC-MS/Thanatochemistry引用本文复制引用
LIU Zhi-wei,MENG Han,LIU Ya-yun,HU Han-qi,SUN Song-po,PEI Yi-ming,ZHU Fang-cheng,YU Xiao-jun..肝组织氨基酸含量变化推断死亡时间的初步研究[J].湖北医药学院学报,2025,44(6):692-695,702,封4,6.基金项目
湖北省大学生创新训练计划(S202513249010) （S202513249010）
湖北医药学院人才启动金项目(2023QDJZR24) （2023QDJZR24）
