针刺研究2025，Vol.50Issue(12)：1365-1375,11.DOI:10.13702/j.1000-0607.20250327
不同强度电针与手针"上巨虚"干预急性炎性内脏痛大鼠的效应及与α7nAChR/PI3K/AKT通路的关系
Effects of different intensities of electroacupuncture and manual acupuncture at"Shangjuxu"(ST37)on acute inflammatory visceral pain and their relationship with α7nAChR/PI3K/AKT signaling pathway
摘要
Abstract
Objective To compare the effects of electroacupuncture(EA)and manual acupuncture(MA)at different stimulation intensities on acute inflammatory visceral pain,and to analyze their mechanisms underlying relief of inflammatory visceral pain mediated by α7 nicotinic acetylcholine receptor(α7nAChR)/phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway.Methods Male SD rats were randomly divided into normal control,visceral pain model,EA 1 mA,EA 2 mA,EA 3 mA,EA 4 mA,EA 6 mA,and MA groups(with the same frequency as EA,2 times/s,for 2 min),with 12 rats in each group.The acute inflammatory visceral pain model was established by intracolonic instillation of 2%acetic acid.EA(2 Hz,1,2,3,4,6 mA)or MA(120 twirlings/min,with the needle rotated at a magnitude of about 90°—180°)was applied to the right"Shangjuxu"(ST37)for 2 min.The abdominal withdrawal reflex(AWR)score and external oblique muscle electromyogram amplitude were used to assess the visceral pain reaction.Histopathological changes of the colonic mucosal tissue were detected by H.E.staining.For observing the involvement of colonic α7nAChR in visceral pain relief of both EA and MA,intraperitoneal injection of methylaconitine(MLA,α7nAChR blocker,4 mg/kg)was given to rats of MA+MLA and EA 2 mA+MLA groups(with 6 rats in each group)before each intervention.The contents of serum tumor necrosis factor-α(TNF-ɑ)and interleukin-1β(IL-1β)were detected by enzyme-linked immunosorbent assay(ELISA).The expression levels of α7nAChR,PI3K and AKT in the colon tissue were detected by Western blot.Results In comparison with the normal control group,both the AWR scores and the amplitude of EMG at colorectal dilation(CRD)pressure of 20,40,60 and 80 mmHg were significantly increased in the model group(P<0.05).Compared with the model group,both the AWR scores and the amplitude of EMG at CRD pressure of 20,40,60 and 80 mmHg were significantly decreased in the EA 1 mA,EA 2 mA and MA groups(P<0.05),rather than in the EA 3 mA,EA 4 mA and EA 6 mA groups,suggesting that the higher stimulation intensity of EA had no analgesic effect.H.E.staining showed slightly hyperemic and edematous injury of the colonic mucosa tissue,with obvious inflammatory infiltration and abnormal morphological changes in the model group,which was obviously milder in the injury in the MA and EA 1 mA,EA 2 mA groups,rather than in the EA 3 mA,4 mA and 6 mA groups.In comparison with the normal control group,the contents of serum TNF-ɑ and IL-1β,and expression levels of PI3K and AKT proteins in colon were significantly increased(P<0.05),and the expression of α7nAChR protein was remarkably down-regulated(P<0.05)in the model group.Compared with the model group,the contents of serum TNF-ɑ and IL-1β,and the expression levels of PI3K and AKT proteins were strikingly down-regulated(P<0.05),and the expression of α7nAChR was considerably up-regulated(P<0.05)in both EA 2 mA and MA groups.Moreover,the effects of reducing the corresponding AWR scores at various CRD pressure levels,serum TNF-α and IL-1β contents,and the protein expressions of PI3K and AKT in colon,as well as increasing the protein expression of α 7nAChR in colon in rats of MA and EA 2 mA groups can be inhibited by MLA(P<0.05),a blocker of α7nAChR.Conclusion Both MA and 2 mA EA(not 3,4,6 mA EA)stimulation of ST37 have a similar analgesic effect in acute inflammatory visceral pain rats,which may be related to their function in suppressing the activities of α7nAChR/PI3K/AKT signaling pathway.关键词
手针/电针/急性炎性内脏痛/α7烟碱乙酰胆碱受体/磷脂酰肌醇3-激酶/蛋白激酶B信号通路/上巨虚Key words
Manual acupuncture/Electroacupuncture/Acute inflammatory visceral pain/α7nAChR/PI3K/AKT signaling pathway/Shangjuxu(ST37)引用本文复制引用
CHEN Cai-yi,LIN Yuan-quan,WANG Zhang-ying,HE Ling-ling,WANG Xuan..不同强度电针与手针"上巨虚"干预急性炎性内脏痛大鼠的效应及与α7nAChR/PI3K/AKT通路的关系[J].针刺研究,2025,50(12):1365-1375,11.基金项目
福建省自然科学基金项目(No.2020J01741、2024J01134) （No.2020J01741、2024J01134）
福建中医药大学校管课题(No.X2023017) （No.X2023017）
福建中医药大学针灸学科开放课题(No.XZJ2022002) （No.XZJ2022002）
