针刺研究2025，Vol.50Issue(12)：1396-1403,8.DOI:10.13702/j.1000-0607.20250429
针刺"大椎""百会""水沟"对脑缺血再灌注损伤大鼠缺血半暗带铜死亡的影响
Effect of acupuncture on cuproptosis in the ischemic penumbra of rats with cerebral ischemia-reperfusion injury
摘要
Abstract
Objective To investigate the effect of acupuncture on the expressions of cuproptosis marker proteins-ferredoxin 1(FDX1)and lipoic acid synthase(LIAS)in the ischemic penumbra(IP)of rats with cerebral ischemia-reperfusion injury(CIRI).Methods SD rats were randomly divided into sham operation,model and acupuncture groups(n=15).The CIRI model was established by occlusion of the middle cerebral artery and reperfusion(MCAO/R).In the acupuncture group,"Dazhui"(GV14),"Baihui"(GV20)and"Shuigou"(GV26)were punctured with filiform needles for 30 min,once every 12 h for 7 times.Neurological function was assessed using modified neurological severity scores(mNSS).Cerebral infarction volume was measured by TTC staining.Copper ion content in IP tissue was determined by colorimetric method.Mitochondrial respiratory chain complexes(MRCC)Ⅰ and Ⅱ contents were detected by ELISA.Protein expressions of FDX1,LIAS,and heat shock protein 70(HSP70)were analyzed by Western blot.Mitochondrial morphology and structure in the IP region were observed via transmission electron microscopy(TEM).Results Compared with the sham operation group,the mNSS score(P<0.01),the volume percentage of cerebral infarction(P<0.000 1),copper ion in IP(P<0.01),MRCCⅠ and Ⅱ contents(P<0.000 1)and HSP70 protein expression(P<0.001)were significantly increased,while the expression of FDX1(P<0.01)and LIAS(P<0.001)were decreased in the model group.Compared with the model group,the mNSS score(P<0.05),the volume percentage of cerebral infarction(P<0.001),the contents of IP copper ion(P<0.001),MRCCⅠ and Ⅱcontents(P<0.01,P<0.000 1)and HSP70 protein expression decreased(P<0.01),whereas the protein expressions of FDX1 and LIAS were increased(P<0.05)in the acupuncture group.TEM results showed that the outer membrane of mitochondria swelled and ruptured,the inner cristae ruptured and disappeared,and the density of the mitochondrial matrix decreased in the model group,which were relatively milder in the acupuncture group.Conclusion Acupuncture intervention can reduce the volume of cerebral infarction and improve the symptoms of neurological deficit in CIRI rats,and its mechanism may be related to reducing the loss of FDX1 and LIAS proteins and inhibiting the surge of HSP70 protein,thus preventing cuproptosis in brain cells.关键词
针刺/脑缺血再灌注损伤/铜死亡/铁氧化还原蛋白1/硫辛酸合酶Key words
Acupuncture/Cerebral ischemia-reperfusion injury/Cuproptosis/FDX1/LIAS引用本文复制引用
LI Qian,XIAO Lu-bo,LUO Xiao-jing,TIAN Chu-ning,HU Shuan,PENG Jia-li,TIAN Hao-mei,LU Xiao-ye,ZHANG Hong..针刺"大椎""百会""水沟"对脑缺血再灌注损伤大鼠缺血半暗带铜死亡的影响[J].针刺研究,2025,50(12):1396-1403,8.基金项目
湖南省自然科学基金项目(No.2024JJ6086) （No.2024JJ6086）
湖南省自然科学基金面上项目(No.S2024JJMSXM2899) （No.S2024JJMSXM2899）
长沙市科技局自然科学基金项目(No.kq2403172) （No.kq2403172）
湖南省中医药科研计划项目(No.B2024131) （No.B2024131）
湖南中医药大学研究生创新项目(No.2024CX032) （No.2024CX032）
