针刺研究2025，Vol.50Issue(12)：1404-1411,8.DOI:10.13702/j.1000-0607.20241069
电针对帕金森病小鼠中脑黑质胰岛素信号转导的影响
Effect of electroacupuncture on insulin signaling in the substantia nigra of the midbrain in mice with Parkinson's disease
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)at"Fengfu"(GV16),"Taichong"(LR3)and"Zusanli"(ST36)on the behavior and insulin receptor substrate 1(IRS-1)/phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)pathway mediated by the insulin-like growth factor 1 receptor(IGF-1R)in the substantia nigra of the midbrain in mice model with Parkinson's disease(PD),so as to explore the mechanism of EA in the prevention and treatment of PD.Methods C57BL/6 mice were randomly divided into a control group,a model group,and an EA group,with 10 mice in each group.A PD mouse model was established by intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP,30 mg·kg-1·d-1)for 7 consecutive days.The mice in the EA group were given EA at GV16,LR3,and ST36 for 30 min each time,once a day for 12 consecutive days.The open field test was used to observe the behavioral changes of the mice in each group before and after treatment.Immunohistochemistry was used to detect the expression level of tyrosine hydroxylase(TH)in the substantia nigra region of the midbrain.Real-time fluorescence quantitative PCR and Western blot were used to detect the mRNA expression levels of IGF-1R,IRS-1,PI3K,and AKT,and protein expressions of IGF-1R,IRS-1,p-PI3K,and p-AKT in the substantia nigra of the mice,respectively.ELISA was used to detect the contents of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in the substantia nigra of the midbrain.Results Compared with the control group,mice in the model group showed obvious motor dysfunction.The total distance of spontaneous movement,the average speed,and the duration of movement,the positive expression level of TH in the substantia nigra of the midbrain,and the mRNA expression levels of IGF-1R,PI3K,and AKT,and protein expressions of IGF-1R,p-PI3K/PI3K and p-AKT/AKT ratio in the substantia nigra decreased(P<0.01,P<0.05),while the mRNA and protein expression levels of IRS-1,the contents of the inflammatory factors TNF-α and IL-1β increased(P<0.05,P<0.01).Compared with the model group,all the above indexes of the PD mice after EA treatment were all reversed(P<0.01,P<0.05).Conclusion EA at GV16,LR3,and ST36 can effectively improve the bradykinesia of PD model mice induced by MPTP,increase the expression level of TH in the substantia nigra,and improve the insulin signaling dysfunction.Its mechanism may be related to the regulation of the IGF-1R/IRS-1/PI3K/AKT signaling pathway and the subsequent inhibition of the neuroinflammatory response.关键词
帕金森病/电针/神经炎性反应/胰岛素样生长因子1受体/磷脂酰肌醇3-激酶/蛋白激酶BKey words
Parkinson's disease/Electroacupuncture/Neuroinflammatory response/Insulin-like growth factor 1 receptor/Phosphatidylinositol 3-kinase/protein kinase B引用本文复制引用
HU Meng-ni,MA Jun,WEI Jia-yong,WANG Yao,LI Ya-nan,ZHU Shu-xiu..电针对帕金森病小鼠中脑黑质胰岛素信号转导的影响[J].针刺研究,2025,50(12):1404-1411,8.基金项目
国家自然科学基金项目(No.81473788) （No.81473788）
中国博士后科学基金项目(No.2023M741110) （No.2023M741110）
