中国临床药理学杂志2025，Vol.41Issue(19)：2767-2773,7.DOI:10.13699/j.cnki.1001-6821.2025.19.012
穿心莲内酯调节miR-218-5p/SOX5影响口腔鳞状细胞癌细胞上皮间质转化的研究
Research of andrographolide modulates epithelial-mesenchymal transition in oral squamous cell carcinoma by miR-218-5p/SOX5 pathways
摘要
Abstract
Objective To investigate the effects of andrographolide on microRNA(miR)-218-5p/RY box transcription factor 5(SOX5)on the proliferation,invasion and epithelial mesenchymal transformation(EMT)of oral squamous cell carcinoma cells.Methods CAL-27 cells were divided into the following groups:blank group(conventional culture),andrographolide group(treated with 15 μmnol·L-1 andrographolide),andrographolide+inhibitor NC group(treated with 15 μmol·L-1 andrographolide and transfected with inhibitor NC),andrographolide+miR-218-5p inhibitor group(treated with 15 μmol·L-1 andrographolide and transfected with miR-218-5p inhibitor),andrographolide+miR-218-5p inhibitor+si-NC group(treated with 15 μmol·L-1 andrographolide and co-transfected with miR-218-5p inhibitor and si-NC),and andrographolide+miR-218-5p inhibitor+si-SOX5 group(treated with 15 μmol·L-1 andrographolide and co-transfected with miR-218-5p inhibitor and si-SOX5).The relative expression levels of miR-218-5p and SOX5 mRNA were detected by quantitative reverse transcription polymerase chain reaction(qRT-PCR),cell proliferation ability was measured using the 5-ethynyl-2′-deoxyuridine(EdU)assay,cell invasion ability was assessed by Transwell assay,and the relative expression levels of EMT-related proteins were determined by Western blotting.Results After different treatment periods,the relative expression levels of miR-218-5p in blank group,andrographolide group,andrographolide+inhibitor NC group and andrographolide+miR-218-5p inhibitor group were 1.00±0.11,2.76±0.48,2.69±0.50 and 1.19±0.17,respectively;the relative expression levels of SOX5 mRNA were 1.00±0.09,0.36±0.07,0.35±0.05 and 0.93±0.18,respectively.Compared with the blank group,the andrographolide group showed statistically significantly upregulated miR-218-5p relative expression levels and statistically significantly reduced SOX5 mRNA relative expression levels(all P＜0.05).The proliferation rates in blank group,andrographolide group,andrographolide+inhibitor NC group,andrographolide+miR-218-5p inhibitor group,andrographolide+miR-218-5p inhibitor+si-NC group and andrographolide+miR-218-5p inhibitor+si-SOX5 group were(62.76±8.14)％,(23.58±2.87)％,(25.12±3.64)％,(51.29±9.12)％,(50.76±8.29)％and(34.18±6.83)％,respectively;the number of invasive cells were(154.23±17.24),(67.13±9.37),(72.81±11.28),(125.36±18.74),(118.15±19.80)and(80.44±12.13),respectively;the relative expression levels of E-cadherin were 1.00±0.12,2.62±0.43,2.56±0.47,1.47±0.27,1.51±0.23 and 2.24±0.43,respectively;the relative expression levels of N-cadherin were 1.00±0.11,0.41±0.08,0.39±0.06,0.83±0.17,0.86±0.15 and 0.51±0.10,respectively;the relative expression levels of Vimentin were 1.00±0.08,0.49±0.07,0.46±0.09,0.85±0.16,0.88±0.17 and 0.55±0.11,respectively.Compared with blank group,andrographolide group showed statistically significantly decreased proliferation rate,number of invasive cells and relative expression levels of N-cadherin and Vimentin,while demonstrating statistically significantly increased relative expression levels of E-cadherin(all P＜0.05).Conclusion Andrographolide inhibits the proliferation,invasion,and EMT in oral squamous cell carcinoma,potentially through upregulating miR-218-5p and subsequently targeting the suppression of SOX5 expression.关键词
穿心莲内酯/口腔鳞状细胞癌/增殖/侵袭/上皮间质转化Key words
andrographolide/oral squamous cell carcinoma/proliferation/invade and attack/epithelial mesenchymal transformation分类
医药卫生引用本文复制引用
ZHANG Fang-ying,HUANG Xin-bang,HUANG Di,ZENG Yi-wang,HUANG Gen-sheng..穿心莲内酯调节miR-218-5p/SOX5影响口腔鳞状细胞癌细胞上皮间质转化的研究[J].中国临床药理学杂志,2025,41(19):2767-2773,7.基金项目
江西省卫生健康委科技计划基金资助项目(SKJP220236690) （SKJP220236690）
