中国临床药理学杂志2025，Vol.41Issue(20)：2906-2911,6.DOI:10.13699/j.cnki.1001-6821.2025.20.010
小檗碱通过miR-125a调控PI3K/AKT信号改善肥胖小鼠胰岛素抵抗和肝脂质代谢的研究
Research of berberine improving insulin resistance and liver fat metabolism in obesity mice by regulating PI3K/AKT signaling through miR-125a
摘要
Abstract
Objective To explore the molecular mechanism of berberine in improving insulin resistance and liver lipid metabolism in obesity mice.Methods Sixty clean-grade C57BL/6 mice were randomly divided into control group(normal diet),model group(high fat diet),experimental-L group(model+50.00 mg·kg-1 berberine),experimental-H group(model+100.00 mg·kg-1 berberine),inhibitor NC group(model+100.00 mg·kg-1 berberine+tail vein injection mmu-inhibitor NC),miR-125 a inhibitor group(model+100.00 mg·kg-1berberine+tail vein injection of mmu-miR-125 a inhibitor).Berberine was administered by gavage once a day,and subcutaneous injection was performed every 4 days.After a continuous treatment period of 28 days,the insulin resistance levels were detected,serum lipid levels were analyzed,the expression levels of related genes were detected by real-time quantitative polymerase chain reaction(RT-qPCR),and the expression of related proteins in liver tissue was detected by Western blot(WB).Results The levels homeostatis model assessment of insulin resistance(HOMA-IR)in control group,model group,experimental-H group,inhibitor NC group and miR-125a inhibitor group were 2.56±0.11,7.23±0.67,5.04±0.20,4.99±0.34 and 6.46±0.40,respectively;triglyceride(TG)levels were(0.46±0.04),(0.93±0.08),(0.65±0.05),(0.63±0.06)and(0.74±0.06)mmol·L-1,respectively;the relative expression levels of miR-125a were 1.00±0.12,0.56±0.07,0.77±0.10,0.78±0.11 and 0.21±0.03,respectively;fatty acid synthase(FAS)mRNA were 1.00±0.10,1.90±0.14,1.40±0.22,1.41±0.16 and 1.67±0.14,respectively;the relative protein expression levels of phosphorylated phosphatidylase 3-kinase(p-PI3K)were 0.78±0.09,0.42±0.05,0.69±0.06,0.70±0.03 and 0.51±0.06,respectively;phosphorylated protein kinase B(p-AKT)protein levels were 0.84±0.12,0.30±0.05,0.64±0.08,0.65±0.08 and 0.49±0.07,respectively.Compared between the model group and the control group;between the experimental-H group and the model group;and between the miR-125a inhibitor group and the inhibitor NC group,all differences of the above inclicatovs went statistically significant(P＜0.01,P＜001).Conclusion Berberine may improve insulin resistance and fat metabolism in obesity mice by activating PI3K/AKT signal through miR-125a.关键词
小檗碱/肥胖/胰岛素抵抗/脂质代谢/微小核糖核酸-125aKey words
berberine/obesity/insulin resistance/fat metabolism/micro ribonucleic acid-125a分类
医药卫生引用本文复制引用
MENG Ke,YIN Dong,LI Ya-bing,LI Jia-rui,DU Li-kun..小檗碱通过miR-125a调控PI3K/AKT信号改善肥胖小鼠胰岛素抵抗和肝脂质代谢的研究[J].中国临床药理学杂志,2025,41(20):2906-2911,6.基金项目
黑龙江省自然科学基金项目(LH2020H085) （LH2020H085）
