中国免疫学杂志2025，Vol.41Issue(12)：2824-2830,7.DOI:10.3969/j.issn.1000-484X.2025.12.002
大麻二酚激活Nrf2-Keap1信号通路抑制TBI大鼠氧化应激反应
Cannabidiol activates Nrf2-Keap1 signaling pathway to inhibit oxidative stress in TBI rats
摘要
Abstract
Objective:To investigate the protective effect of cannabidiol(CBD)against oxidative stress in rats with traumatic brain injury(TBI)and its relationship with Nrf2-Keap1 signaling pathway.Methods:Adult male SD rats were randomly divided into sham-operated group(Sham),model group(TBI+vehicle),CBD-intervention group(TBI+CBD)and pathway inhibitor group(TBI+CBD+ML385).Modified Feeney free-fall method was used to establish TBI model rats,pathological changes,oxidative stress and inflammation in rat brain tissue were observed by combination of neurological deficit scoring(mNSS),HE staining,immunofluores-cence staining,Western blot,RT-qPCR and ELISA.Results:mNSS score was significantly increased and pathological changes such as abnormal neuronal cell morphology and inflammatory cell infiltration were observed in model group,IL-1β and TNF-α positive expressions were significantly enhanced,GSH content and Nrf2 protein expression were significantly reduced,ROS,MDA contents and Keap1 mRNA were significantly increased.mNSS score was significantly reduced in TBI+CBD group,pathological condition of brain tissue was improved,IL-1β,TNF-α positive expressions were reduced,GSH content and Nrf2 expression were significantly increased,ROS,MDA contents and Keap1 mRNA were significantly decreased,and ML385 could significantly inhibit antioxidant effect of CBD.Conclusion:CBD can inhibit oxidative stress after TBI through Nrf2-Keap1 pathway,reduce secondary pathological injury in TBI rats,and play a certain neuroprotective role.关键词
创伤性脑损伤/大麻二酚/氧化应激/Nrf2-Keap1信号通路Key words
Traumatic brain injury/Cannabidiol/Oxidative stress/Nrf2-Keap1 signaling pathway分类
医药卫生引用本文复制引用
ZHU Juan,LI Ping,LI Hengxi,LI Jiali,LING Tenghan,CAO Yan,YIN Aiping,MA Yuan,GUO Xiaobing,WU Haiying..大麻二酚激活Nrf2-Keap1信号通路抑制TBI大鼠氧化应激反应[J].中国免疫学杂志,2025,41(12):2824-2830,7.基金项目
国家自然科学基金地区科学基金(82060241,82260387,82560263) （82060241,82260387,82560263）
云南省基础研究计划项目-重点项目(202501AS070026) （202501AS070026）
云南省教育厅科学研究基金(2023J0217) （2023J0217）
云南省科技厅科技计划基础研究专项(202301AS070020) （202301AS070020）
云南省科技厅昆明医科大学应用基础研究联合专项(202101AY070001-037) （202101AY070001-037）
国家大学生创新训练项目(202210678029). （202210678029）
