中国免疫学杂志2025，Vol.41Issue(12)：2867-2871,5.DOI:10.3969/j.issn.1000-484X.2025.12.009
IL-35对氧糖剥夺/复氧复糖诱导的BV2细胞损伤的保护作用
Protective effect of IL-35 for oxygen-glucose deprivation/reperfusion induced injury in BV2 cells
摘要
Abstract
Objective:To investigate protective effect of IL-35 against oxygen-glucose deprivation/reperfusion(OGD/R)-in-duced microglia BV2 injury and possible mechanism.Methods:BV2 cells were cultured in vitro,and experiment was divided into Control group,OGD/R group and IL-35 group;CCK-8 was used to detect cell viability,colorimetric assay was used to detect cellular oxidative stress level,ELISA was used to detect expressions of inflammatory factors,high-throughput sequencing was used to analyze differential genes,PCR and Western blot were used to detect expressions of cGAS and STING gene and protein.Results:Compared with OGD/R group,viability of BV2 cells in IL-35 group was significantly increased,expressions of ROS,MDA,IL-6,IL-1β and TNF-α in IL-35 group were significantly decreased,while expression of SOD was significantly increased.Differential gene analysis showed that between OGD/R group and IL-35 group,there were 290 up-regulated genes and 261 down-regulated genes;KEGG enrich-ment analysis showed that down-regulated genes were mainly enriched in cytoplasmic DNA sensing pathway.PCR and Western blot results showed that cGAS and STING gene and protein expressions in IL-35 group were significantly lower than OGD/R group(all P<0.05).Conclusion:IL-35 has a protective effect on OGD/R-induced BV2 cell damage,and its mechanism may be related to cGAS-STING signalling pathway.关键词
IL-35/氧糖剥夺/复氧复糖/小胶质细胞BV2/cGAS/STINGKey words
IL-35/Oxygen-glucose deprivation/reperfusion/Microglia BV2/cGAS/STING分类
医药卫生引用本文复制引用
LI Ming,YU Lulu,ZHOU Hai,YANG Shengkai,GUO Weiwei..IL-35对氧糖剥夺/复氧复糖诱导的BV2细胞损伤的保护作用[J].中国免疫学杂志,2025,41(12):2867-2871,5.基金项目
南京医科大学康达学院2022年度科研发展基金课题(KD2022KYJJZD159). （KD2022KYJJZD159）
