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基于eDNA宏条形码技术的莱州湾海洋牧场鱼类多样性研究

WU Sihang YAN Pengjin AN Changting LI Ang LI Jiao CHE Shuai LIU Chenglei LIU Shufang

中国水产科学2025，Vol.32Issue(9)：1327-1343,17.
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中国水产科学2025，Vol.32Issue(9)：1327-1343,17.DOI:10.12264/JFSC2025-0082

基于eDNA宏条形码技术的莱州湾海洋牧场鱼类多样性研究

Study on fish diversity in Laizhou Bay marine ranch based on eDNA metabarcoding

WU Sihang 1YAN Pengjin 2AN Changting 3LI Ang 3LI Jiao 3CHE Shuai 3LIU Chenglei 4LIU Shufang3

作者信息

  • 1. College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China||Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences||Qingdao 266071,China
  • 2. Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences||Qingdao 266071,China||College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China
  • 3. Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences||Qingdao 266071,China
  • 4. Shandong Mingbo Seed Industry Technology,Yantai 261441,China
  • 折叠

摘要

Abstract

This study employed environmental DNA(eDNA)metabarcoding technology with a local 12S rDNA database of Yellow and Bohai Sea fishes to monitor fish diversity in Jincheng Marine Ranch,Laizhou Bay.Key findings include:(1)84 fish species significantly surpassed the four species recorded during baseline surveys using gillnets,traps,and longlines in 2009 during ranch construction,demonstrating the superior sensitivity of eDNA technology and providing reference data for the diversity of fish;(2)41 species(48.81%of eDNA findings,50.62%of historical records)overlapped with historical Laizhou Bay records,while the remaining 43 species represent Yellow-Bohai Sea records.Discrepancies with historical records arise from methodological differences in species detection efficiency,the unique location of the ranch,and interannual variations in fish distribution;(3)Seasonal variations revealed maximum diversity in winter(60 species)and minimum in summer(30 species),with 12 species present year-round.Alpha diversity analysis showed higher diversity in autumn and summer than in winter and spring.Beta diversity indicated greater seasonal community differences than spatial variations within seasons.(4)The local database,despite having lower species coverage,demonstrated superior annotation accuracy compared with the NCBI database,thereby effectively eliminating non-target species interference and correcting misannotations for five species.The eDNA approach overcomes traditional limitations in reef areas(low catch efficiency and gear damage)while maintaining ecological integrity through non-invasiveness.This technique enables comprehensive monitoring of diverse ecological types and behavioral habits,providing robust data support for biodiversity conservation management in Laizhou Bay and effective technical evaluation for marine ranch restoration,thereby facilitating scientific management and sustainable development of marine ranching.

关键词

eDNA宏条形码/鱼类多样性/莱州湾/海洋牧场/12S rDNA本地数据库

Key words

eDNA metabarcoding/fish diversity/Laizhou Bay/marine ranch/12S rDNA local data base

分类

农业科技

引用本文复制引用

WU Sihang,YAN Pengjin,AN Changting,LI Ang,LI Jiao,CHE Shuai,LIU Chenglei,LIU Shufang..基于eDNA宏条形码技术的莱州湾海洋牧场鱼类多样性研究[J].中国水产科学,2025,32(9):1327-1343,17.

基金项目

国家重点研发计划项目(2024YFD2400401) （2024YFD2400401）

现代农业产业技术体系项目(CARS-47) （CARS-47）

中央级公益性科研院所基本科研业务费专项(20603022024023). （20603022024023）

中国水产科学

OA北大核心

1005-8737

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