中国水产科学2025，Vol.32Issue(10)：1404-1417,14.DOI:10.12264/JFSC2025-0175
牙鲆pmela基因的序列及其在体色形成中的表达特征
Sequence of pmela gene and its expression characteristics of body color formation in Paralichthys olivacaus
摘要
Abstract
We investigated the sequence characteristics of the pmela gene in Japanese flounder(Paralichthys olivaceus)and its expression pattern in body color formation by cloning the CDS region of the pmela gene and analyzing its protein properties using bioinformatics.The expression profiles during larval pigment formation in different juvenile tissues and colored adult skin were analyzed via quantitative real-time PCR(qRT-PCR)and immunohistochemistry.The results showed that the CDS region of pmela was 2445 bp in length,encoded 814 amino acids,and contained a PKD domain and transmembrane region.Phylogenetic analysis revealed its closest evolutionary relationship with Pleuronectiformes species,sharing over 80％homology,and classified it as a stable hydrophobic protein.QRT-PCR analysis showed that during larval development,pmela mRNA expression levels differed synchronously with pigment deposition,indicating an initial upregulation followed by a later downregulation.Among different tissues,pmela was predominantly highly expressed in the eyes and skin.In adult skin,its expression levels in both gray and black skin were significantly higher than those in white skin(P＜0.05).Immuno-histochemical analysis revealed that the Pmela protein was primarily enriched in the retinal pigment epithelium and the basal layer of the skin,with the highest distribution in black skin.The findings of this study confirm that the pmela gene participates in body color formation in Japanese flounder by regulating melanosome maturation.It is hypothesized that pmela may play a key role in the early stages of melanosome formation,laying a preliminary research foundation for obtaining golden-colored Japanese flounder through pmela gene editing.关键词
pmela/基因克隆/生物信息学/色素发育/牙鲆Key words
pmela/gene cloning/bioinformatics/pigment development/Paralichthys olivaceus分类
农业科技引用本文复制引用
TAO Yuehong,HOU Jilun,REN Yuqin,WANG Guixing,ZHANG Yitong,YANG Yucong,WANG Xiyuan,HE Zhongwei,LIU Yufeng,CAO Wei..牙鲆pmela基因的序列及其在体色形成中的表达特征[J].中国水产科学,2025,32(10):1404-1417,14.基金项目
国家现代农业产业技术体系项目(CARS-47) （CARS-47）
河北省重点研发计划现代种业科技创新专项(21326307D) （21326307D）
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金项目(2023TD41,2022XT0304). （2023TD41,2022XT0304）
