中国水产科学2025，Vol.32Issue(10)：1431-1444,14.DOI:10.12264/JFSC2025-0096
添加亚硝酸盐对生物絮团硝化作用建立及硝化性能的影响
Effects of nitrite addition on the establishment and performance of nitrification in bioflocs
摘要
Abstract
Nitrite-oxidizing bacteria(NOB)growth is often inhibited by high ammonia nitrogen and low nitrite concentrations during conventional nitrification,delaying system establishment.In this study,nitrite was supplemented at the onset of biofloc nitrification to promote NOB growth,and its effects on nitrification establishment,nitrification performance,nutrient composition,and microbial community structure were evaluated.Initial nitrite nitrogen(NO2--N)concentrations in the biofloc cultivation system were set at 0,2,10,50,and 100 mg/L(groups N-0,N-2,N-10,N-50,and N-100,respectively).The results showed that NO2--N in the biofloc cultivation systems of groups N-50 and N-100 decreased more rapidly than those in other groups.The nitrification establishment time was 27 days,which was significantly shorter than that of other groups(P＜0.05).The ammonia nitrogen treatment capacity of group N-100 was(0.77±0.05)mg/(g·h),which was significantly higher than that of other groups(P＜0.05).No significant differences were observed among the groups in crude fat,crude protein,crude ash,fatty acids,and amino acids(P＞0.05).The microbial communities in groups N-50 and N-100 differed significantly from those of other groups:the abundance of Proteobacteria in groups N-50 and N-100 was significantly decreased,whereas the abundance of Actinobacteria and Chloroflexi was significantly increased(P＜0.05).The relative abundances of Paracoccus and norank_f__Alcaligenaceae in groups N-50 and N-100 were significantly lower than those in other groups,whereas the relative abundances of Glutamicibacter and Comamonas were significantly higher than those in other groups(P＜0.05).The gene abundances of ammonia monooxygenase(amoA)and hydroxylamine oxidase(hao)in groups N-50 and N-100 were significantly higher than those in other experimental groups.The gene abundance of nitrite oxidase(nxrA)in nitrite-added groups was significantly elevated(P＜0.05),resulting in an enhanced nitrite oxidation capacity.The findings demonstrate that supplementing nitrite to achieve an initial NO2--N concentration of 100 mg/L during biofloc cultivation significantly accelerates nitrification establishment and improves biofloc nitrification performance.关键词
生物絮团/亚硝酸盐氧化细菌/亚硝酸盐/硝化作用Key words
biofloc/nitrite oxidizing bacteria/nitrite/nitrification分类
农业科技引用本文复制引用
TANG Yixin,HU Xin,SUN Gao,GUO Yanshuo,TAN Hongxin,LUO Guozhi..添加亚硝酸盐对生物絮团硝化作用建立及硝化性能的影响[J].中国水产科学,2025,32(10):1431-1444,14.基金项目
国家自然科学基金项目(32273187). （32273187）
