中国实验动物学报2025，Vol.33Issue(11)：1559-1573,15.DOI:10.3969/j.issn.1005-4847.2025.11.001
类风湿关节炎血瘀证大鼠模型的构建及评价
Establishment and evaluation of a rat model of blood stasis syndrome in rheumatoid arthritis
摘要
Abstract
Objective To construct collagen-induced arthritis(CIA)rat models of different blood stasis syndrome types and evaluate various modeling method,thereby providing a basis for establishing stable and reliable rat models of blood stasis syndrome in rheumatoid arthritis(RA).Methods Based on the CIA rat model,six different blood stasis models were established by superimposing various factors including adrenaline injection,ice-water bath stimulation,starvation,lipopolysaccharide(LPS)administration,and high-fat diet feeding.The modeling method were comprehensively evaluated by measuring joint swelling,arthritis index(AI)scoring,Micro-CT,hematoxylin and eosin(HE)staining,as well as assessing blood routine,hemodynamic parameters,and detecting inflammatory markers,coagulation indicators,and blood stasis-related indices via ELISA.Results Compared with the control group,the CIA-only group showed significantly increased inflammatory markers,hematological parameters,hemodynamic indices,and coagulation indicators.Micro-CT revealed significant bone destruction,and HE staining indicated rough articular surfaces and inflammatory cell infiltration.On this basis,different superimposed stimuli aggravated blood stasis-related manifestations to varying degrees:CIA+Adr group and CIA+LPS group exhibited comprehensive increases in inflammation,coagulation,and vasoactive substances.The CIA+Adr+ice water bath group showed worsened inflammation along with partial decreases in hemodynamic parameters.The CIA+Adr+ice water bath+starvation group demonstrated abnormalities in inflammatory and coagulation markers alongside reductions in certain factors.The CIA+LPS+high-fat diet group and CIA+high-fat diet group primarily exhibited abnormalities in hematological and vascular endothelial indicators.Conclusions Multiple stimuli can successfully induce different blood stasis syndrome types in CIA model rats,with certain differences observed.This study provides a comprehensive multidimensional evaluation of various modeling method for RA blood stasis syndrome,offering an experimental basis for further research into the pathogenesis and treatment strategies of this disease pattern.关键词
类风湿关节炎/血瘀证/动物模型/造模方法/模型评价Key words
rheumatoid arthritis/blood stasis syndrome/animal model/modeling method/model evaluation分类
生物科学引用本文复制引用
CHEN Meiqi,HE Yiting,HE Xiaohong,CHEN Bozhen,MA Mengyang,YANG Lu,WU Jiaqi,ZHOU Yingyan,HUANG Runyue,WANG Maojie,HUANG Qingchun..类风湿关节炎血瘀证大鼠模型的构建及评价[J].中国实验动物学报,2025,33(11):1559-1573,15.基金项目
国家自然科学基金(82174285),广东省中医药防治难治性慢病重点实验室项目(2023KT15525),广东省基础与应用基础研究基金项目(2020B1515130005),广东省名中医工作室建设项目(0102018004). Funded by National Natural Science Foundation of China(82174285),Guangdong Provincial Key Laboratory Project of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases(2023KT15525),Guangdong Basic and Applied Basic Research Foundation(2020B1515130005),Guangdong Provincial Famous Traditional Chinese Medicine Practitioner Studio Construction Project(0102018004). （82174285）
