中国实验动物学报2025，Vol.33Issue(11)：1619-1628,10.DOI:10.3969/j.issn.1005-4847.2025.11.006
酒黄精多糖对AD果蝇模型运动、记忆和抗氧化能力影响的研究
Effects of polysaccharides from wine-processed Polygonatum sibiricum on movement,memory and antioxidant capabilities of Alzheimer's disease transgenic Drosophila melanogaster
摘要
Abstract
Objective To investigate the effects of polysaccharides from wine-processed Polygonatum sibiricum(PSPW)on the behavioral performance and antioxidant indices of Alzheimer's disease(AD)transgenic D.melanogaster,and evaluate its therapeutic effect on AD.Methods Amyloid β42(Aβ42)transgenic D.melanogaster were divided randomly into model,and low,medium,and high dose PSPW groups.w1118 D.melanogaster with the same genetic background as the control group.After 28 d of intervention,the lifespan,climbing ability,and olfactory memory were evaluated systematically.Aβ1-42 and malondialdehyde(MDA)levels in brain tissue,superoxide(SOD)and catalase(CAT)activities,and the expression levels of antioxidant genes in the Keap1/Nrf2 signaling pathway were detected by ELISA and qPCR.Results Behavioral studies showed that AD transgenic D.melanogaster had a significantly shorter lifespan and significantly impaired climbing and olfactory memory abilities compared with the control group.PSPW significantly improved the above behavioral indicators compared with the model group.Regarding antioxidant indicators,brain tissue levels of Aβ1-42 and MDA levels were significantly increased in the model group,while SOD and CAT activities were significantly decreased.Medium and high dose PSPW group significantly reduced Aβ1-42 and MDA levels,increased SOD and CAT activities,and enhanced antioxidant capacity,while D.melanogaster in the low-dose PSPW group showed an improved but non-significant trend.Detection of antioxidant genes in the Kelch-like ECH-associated protein 1(Keap1)/nuclear factor erythroid 2-related factor 2(Nrf2)pathway showed that Keap1 expression was increased in the model group,while Nrf2,gamma-glutamylcysteine synthetase(GCL),and glutathione S-transferase S1(gsts1)expression were decreased.High dose PSPW group significantly down-regulated Keap1 and up-regulated Nrf2,GCL,and gsts1 expression,while the medium and low dose PSPW groups showed an activation trend but no significant difference.Conclusions PSPW regulate the Keap1/Nrf2 antioxidant pathway,enhance antioxidant enzyme activities,reduce the accumulation of oxidative products,and achieve the therapeutic effects of reducing Aβ deposition in AD transgenic D.melanogaster and improving motor and memory dysfunctions.关键词
酒黄精多糖/阿尔茨海默病/AD果蝇模型/行为学/抗氧化Key words
polysaccharides from wine-processed Polygonatum sibiricum/Alzheimer's disease/AD transgenic D.melanoyaster/behavior studies/antioxidation分类
生物科学引用本文复制引用
WU Li,WANG Shuaikang,ZENG Jialing,ZHONG Xi,MU Jiaxin,HUANG Liping..酒黄精多糖对AD果蝇模型运动、记忆和抗氧化能力影响的研究[J].中国实验动物学报,2025,33(11):1619-1628,10.基金项目
江西省教育厅科技计划(GJJ2400817),江西省自然科学基金(20242BAB26166),中药药理研究江西省重点实验室省科技创新基地计划-省重点实验室(2024SSY07111),江西省研究生创新专项(YC2024-B220),中药科研实践创新训练项目(24KYCX-ZD004). Funded by Jiangxi Provincial Department of Education Science and Technology Program(GJJ2400817),Jiangxi Provincial Natural Science Foundation(20242BAB26166),Jiangxi Provincial Key Laboratory of Traditional Chinese Medicine Pharmacology Provincial Science and Technology Innovation Base Program-Provincial Key Laboratory(2024SSY07111),Jiangxi Provincial Graduate Student Innovation Special Project(YC2024-B220),Traditional Chinese Medicine Scientific Research Practice Innovation Training Project(24KYCX-ZD004). （GJJ2400817）
