中国实验动物学报2025，Vol.33Issue(11)：1629-1635,7.DOI:10.3969/j.issn.1005-4847.2025.11.007
多房棘球蚴感染导致小鼠中心碳代谢紊乱及免疫细胞糖酵解功能抑制
Echinococcus multilocularis infection induces central carbon metabolism disorders and suppresses glycolytic function in mouse immune cells
摘要
Abstract
Objective To elucidate the metabolic reprogramming effects of Echinococcus multilocularis infection on host energy metabolism and its associated immunoregulatory mechanisms.Methods Serum levels of 57 central carbon metabolites in infected mice were detected using targeted metabolomics technology.The energy metabolic functions of peripheral blood mononuclear cell(PBMC)and splenic CD4+T cells were evaluated via mitochondrial and glycolytic stress tests using the Seahorse XF24 energy metabolism analyzer system.Results Serum levels of L-arginine,succinate,isocitrate,dihydroxyacetone phosphate,and malate were significantly decreased(P＜0.05)in the infected group.Kyoto encyclopedia of genes and genomes analysis revealed significant alterations in five pathways,including central carbon metabolism in cancer.Both mitochondrial respiratory function and glycolytic capacity were suppressed in PBMCs in the infected group(P＜0.05).No significant mitochondrial dysfunction was observed in splenic CD4+T cells,but their glycolytic activity was markedly reduced(P＜0.05).Conclusions E.multilocularis infection disrupts central carbon metabolism in mouse serum and significantly suppresses glycolytic function in both PBMCs and CD4+T cells.These findings suggest that energy metabolic reprogramming may play a critical role in parasite immune evasion.关键词
多房棘球蚴/中心碳代谢/糖酵解/线粒体Key words
Echinococcus multilocularis/central carbon metabolism/glycolysis/mitochondria分类
生物科学引用本文复制引用
WANG Chuan,MU Hu,DAI Zhongshi,ZHU Mingxing..多房棘球蚴感染导致小鼠中心碳代谢紊乱及免疫细胞糖酵解功能抑制[J].中国实验动物学报,2025,33(11):1629-1635,7.基金项目
宁夏自然科学基金(2025AAC030657),宁夏常见传染病防控重点实验室开放课题(2023-KF003,2024-KF003). Funded by the Ningxia Natural Science Foundation(2025AAC030657),the Open Project of Ningxia Key Laboratory of Common Infectious Disease Prevention and Control(2023-KF003,2024-KF003). （2025AAC030657）
