中国沼气2025，Vol.43Issue(6)：29-36,8.DOI:10.20022/j.cnki.1000-1166.2025060029
合成微生物组预处理对蒸汽爆破高粱秸秆厌氧发酵性能的影响研究
Pretreating Steam-Exploded Sorghum Straw with a Synthetic Microbiome to Enhance Anaerobic Fermentation Performance
摘要
Abstract
Pretreatment is a promising strategy for enhancing the anaerobic fermentation performance of sorghum straw.However,the effects and underlying mechanisms of synthetic microbiome pretreatment on the anaerobic fermentation of steam-exploded sorghum straw remain unclear.In this study,a steam-exploded sorghum straw,a by-product of liquor pro-duction,was pretreated with a self-constructed,highly efficient synthetic microbial consortium.We systematically analyzed its impact on anaerobic fermentation performance.The results demonstrated that the synthetic microbiome pretreatment led to significant advantages during the initial fermentation phase,including higher daily and cumulative methane yields,as well as distinct profiles of pH and volatile fatty acids(VFAs).Furthermore,Illumina high-throughput sequencing revealed sig-nificant differences in microbial community structure between the treated and control groups.Specifically,the relative abun-dances of the methanogenic archaea Methanosarcina and Methanobacterium were markedly higher in the experimental group.These findings not only provide a potential technical solution to mitigate the slow start-up issue commonly encountered in the anaerobic fermentation of sorghum straw but also offer a theoretical foundation for the biogas utilization of characteristic agri-cultural wastes,typified by distilled spirit sorghum straw,in regions such as Sichuan and Guizhou.关键词
合成微生物组/高粱秸秆/厌氧发酵/蒸汽爆破/预处理Key words
synthetic microbiome/sorghum straw/anaerobic fermentation/steam explosion/pretreatment分类
农业科技引用本文复制引用
WANG Jun,LIU Linpei,HUANG Yuling,CHEN Xiaoru,REN Yao,MA Yiran,ZHUANG Yong,ZHU Qili,ZHANG Xueping,TAN Furong..合成微生物组预处理对蒸汽爆破高粱秸秆厌氧发酵性能的影响研究[J].中国沼气,2025,43(6):29-36,8.基金项目
四川省区域创新合作项目(2024YFHZ0201) （2024YFHZ0201）
中国农业科学院基础科技创新项目(JCKJ2025-JC-03) （JCKJ2025-JC-03）
固态发酵资源利用四川省重点实验室开放基金项目(2024GTYY01) （2024GTYY01）
四川工商职业技术学院青年专项课题(2024NC08). （2024NC08）
