浙江农林大学学报2025，Vol.42Issue(6)：1142-1153,12.DOI:10.11833/j.issn.2095-0756.20250263
青钱柳GATA基因家族鉴定及盐胁迫下表达分析
Identification of the GATA gene family in Cyclocarya paliurus and expression analysis under salt stress
摘要
Abstract
[Objective]This study aims to investigate the regulatory function of GATA transcription factors in Cyclocarya paliurus under salt stress.[Method]Based on the genomic and transcriptomic data of C.paliurus,members of the GATA gene family were identified,and their physicochemical properties,gene structure,and phylogenetic evolution were comprehensively analyzed.The expression patterns of CpGATAs in response to salt stress were investigated through salt stress treatments with 4 sodium chloride concentrations(0,25.7,51.3 and 77.0 mmol·L-1).[Result]The 46 GATA members were identified from the genome,distributed on 14 chromosomes.The encoded protein had a length of 132-827 amino acids,a molecular weight of 14 358.49-92 724.86 Da,and a hydrophilicity coefficient of-1.119--0.368.Subcellular localization showed that 37 GATA genes were located in the nucleus,while the remaining 9 genes were distributed in chloroplasts,cytoplasm,and plasma membrane.The analysis and prediction of promoter cis acting elements indicated that GATA gene family members included 13 cis-acting promoter elements,including auxin responsive element,gibberellin responsive element and low-temperature responsive element.Transcriptome analysis showed that the expression levels of 12 genes,including CpaF1st31008,CpaF1st45364,and CpaF1st12063,were higher at 30 days of salt treatment than at 15 days,and were upregulated under salt stress.However,18 genes,including CpaF1st13794 and CpaF1st04260,were downregulated under salt stress.[Conclusion]Members of the GATA gene family in C.paliurus actively participate in salt stress response at the transcriptional level and exhibit diverse response patterns.[Ch,8 fig.2 tab.31 ref.].关键词
青钱柳/GATA转录因子/盐胁迫/表达分析Key words
Cyclocarya paliurus/GATA transcription factor/salt stress/expression analysis分类
农业科技引用本文复制引用
ZHANG Lei,CHEN Hui,ZHANG Zijie,SHANG Xulan,YANG Wanxia,FANG Shengzuo..青钱柳GATA基因家族鉴定及盐胁迫下表达分析[J].浙江农林大学学报,2025,42(6):1142-1153,12.基金项目
国家自然科学基金青年基金资助项目(32301570) （32301570）
