浙江农林大学学报2025，Vol.42Issue(6)：1154-1164,11.DOI:10.11833/j.issn.2095-0756.20250103
枳OSCA基因家族全基因组鉴定及干旱胁迫响应分析
Genome-wide identification and drought stress response analysis of the OSCA gene family in Poncirus trifoliata
摘要
Abstract
[Objective]This study aims to reveal the structural characteristics of the OSCA gene family in Poncirus trifoliata and its expression profile under drought stress,providing a reference for PtOSCA gene functional analysis and drought resistance research.[Method]Bioinformatic methods were used to identify and comprehensively analyze PtOSCA gene family members across the whole genome.RT-qPCR was used to analyze the dynamic expression profiles of PtOSCA genes under PEG treatment.[Result]Thirteen PtOSCAs were identified in the P.trifoliata genome and named PtOSCA1-PtOSCA13 according to their homology with Arabidopsis thaliana OSCA genes.Chromosomal localization analysis indicated that these genes were unevenly distributed across 7 out of the 18 chromosomes in P.trifoliata.Phylogenetic analysis showed that PtOSCAs were divided into four groups,with 5,6,1,and 1 family members respectively.Analysis of protein physicochemical properties and conserved domains revealed that there were differences in protein length and molecular weight among PtOSCAs,while the motifs were relatively conserved,with all PtOSCAs proteins containing Motif 1 and Motif 3.Cis-acting element analysis indicated that the promoter regions of PtOSCAs had numerous cis-elements responsive to drought,abscisic acid,anaerobic induction,and low-temperature stress.Gene expression analysis demonstrated that PtOSCAs had tissue-specific expression and responses to drought stress.Under drought stress,the expression levels of PtOSCA family members changed to varying degrees in both leaves and roots.In leaves,11 PtOSCAs were induced by drought stress,and in roots,10 PtOSCAs were upregulated,with PtOSCA4 upregulated 3.76-fold in leaves and 36.74-fold in roots after drought stress induction.[Conclusion]The PtOSCA gene family shows evolutionary conservation and shares a close phylogenetic relationship with the OSCA genes of A.thaliana and Citrus sinensis.Functional differentiation exists within the PtOSCA gene family,and PtOSCA4 may play a key role in drought stress response of P.trifoliata.The results of this study provide an important basis for research on the molecular functions of PtOSCAs under drought stress in P.trifoliata.[Ch,6 fig.2 tab.27 ref.].关键词
枳/柑橘/OSCA基因/干旱胁迫/表达分析Key words
Poncirus trifoliata/Citrus/OSCA/drought stress/expression analysis分类
农业科技引用本文复制引用
LI Jiaxuan,CHEN Yuewen,ZHOU Tie,SHI Jun,YANG Junfeng,LU Xiaopeng,LI Feifei..枳OSCA基因家族全基因组鉴定及干旱胁迫响应分析[J].浙江农林大学学报,2025,42(6):1154-1164,11.基金项目
国家重点研发计划项目(2024YFD2300800) （2024YFD2300800）
国家自然科学基金资助项目(32102313,32172520) （32102313,32172520）
