浙江农林大学学报2025，Vol.42Issue(6)：1192-1200,9.DOI:10.11833/j.issn.2095-0756.20250118
辽宁省杨树叶锈病病原菌的鉴定
Pathogen identification of poplar leaf rust diseases in Liaoning Province
摘要
Abstract
[Objective]Poplar leaf rust diseases,caused by fungi of the genus Melampsora,are one of the most widespread and hamful foliar diseases affecting poplar trees.This study aims to elucidate the occurrence of poplar leaf rust disease and the species diversity of its pathogens at the National Poplar Germplasm Resources Base in Liaoning Province,China.[Method]In October 2024,the 39 samples of poplar leaf rust disease were collected from the National Poplar Germplasm Resources Base in Liaoning Province and Liaoning Provincial Institute of Poplar,China.The morphological characteristics of urediniospores and teliospores of poplar leaf rust disease from the 2 locations were observed using an optical microscope and scanning electron microscope.Meanwhile,the genomic DNA of the pathogen was extracted,and the phylogenetic tree was constructed by rDNA ITS and 28S sequencing analysis.The pathogens were identified through a combination of morphological and molecular phylogenetic analyses.[Result]The results indicated that 36 samples were identified as M.laricis-populina,2 samples were M.medusae,and 1 sample was M.populnea.M.medusae,a significant quarantine species in China,was detected for the first time in Northeastern China.[Conclusion]The 3 species of Melampsora were discoveried in Liaoning.This study also represents the first detection of the exotic quarantine pathogen M.medusae in Northeastern China.[Ch,6 fig.2 tab.38 ref.].关键词
杨树叶锈病/形态学观察/分子检测/栅锈菌属Key words
poplar leaf rust disease/morphological observation/molecular detection/Melampsora分类
农业科技引用本文复制引用
BAI Qian,WANG Zhanbin,FENG Lianrong,WANG Shiqi,LIU Wei,PENG Rusheng..辽宁省杨树叶锈病病原菌的鉴定[J].浙江农林大学学报,2025,42(6):1192-1200,9.基金项目
东北亚生物多样性研究中心项目(411147021003) （411147021003）
辽宁省林业和草原局2024年度依托国家林业和草原局科技创新平台研发项目(LLC[2024]18) （LLC[2024]18）
