针灸推拿医学（英文版）2025，Vol.23Issue(6)：476-484,9.DOI:10.1007/s11726-025-1530-0
基于cAMP/PKA通路探讨"调气明目"穴方对单眼形觉剥夺大鼠视觉可塑性的调节机制
Exploring the regulatory mechanism of"Tiao Qi Ming Mu"point prescription on visual plasticity in monocular vision deprivation rats based on cAMP/PKA pathway
摘要
Abstract
Objective:To investigate the intervention mechanism of acupuncture on visual cortical plasticity in rats with monocular vision deprivation by examining the regulatory effects of"Tiao Qi Ming Mu"(Qi-regulating and eyesight-improving)on the metabotropic glutamate receptor 1(mGluR1)-mediated cyclic-adenosine monophosphate(cAMP)/protein kinase A(PKA)signaling cascade. Methods:Forty-eight Sprague-Dawley rats were randomly divided into a blank group,a model group,a covering group,and an acupuncture group.The deprivation amblyopia model was established by right eyelid suture in all groups except the blank group.In the covering group,the sutured right eye was cut open,and the left eyelid was sutured on the 7th day after modeling.In the acupuncture group,the"Tiao Qi Ming Mu"point prescription was administered starting on the 7th day after model establishment and continued for 14 d.The electrophysiological changes in binocular vision of rats were detected using pattern visual evoked potential(P-VEP);in visual cortex area 17,the mGluR1 protein expression was detected using immunofluorescence staining,the mGluR1 mRNA level was detected by real-time polymerase chain reaction,and the mGluR1,cAMP,and PKA protein levels were detected by Western blotting. Results:Compared to the blank group,the P-VEP's P100 wave latency period delayed,and the amplitude reduced in the vision-deprived eye of the model group,showing statistical significance(P<0.05);the protein and mRNA expression of mGluR1 and the protein expression of cAMP and PKA decreased in visual cortex area 17 in the model group,and the differences were significant(P<0.05).After 14 d of intervention,compared to the model group,the vision-deprived eye's P-VEP waveform improved with advanced P100 wave latency periods and increased amplitudes in the covering group and the acupuncture group,and the differences were significant(P<0.05);the fluorescence expression of mGluR1 protein and the protein and mRNA expression of mGluR1 increased significantly(P<0.05);the cAMP and PKA protein expression increased significantly(P<0.05)in the covering group and the acupuncture group. Conclusion:"Tiao Qi Ming Mu"point prescription can activate the cAMP/PKA signaling pathway mediated by mGluR1 in visual cortex area 17 and promote the synaptic plasticity changes of visual cortex neurons of monocular vision-deprived amblyopia model rats,thereby improving the optic nerve conduction efficiency and promoting the recovery of visual function.关键词
针刺疗法/弱视/形觉剥夺/视皮层/代谢性谷氨酸受体/cAMP/PKA信号通路/大鼠Key words
Acupuncture Therapy/Amblyopia/Vision Deprivation/Visual Cortex/Metabotropic Glutamate Receptors/cAMP/PKA Signal Pathway/Rats分类
医药卫生引用本文复制引用
LIU Anguo,ZHANG Qian,ZHOU Na,KAN Lili,HAO Xiaolu,WU Yanhui,ZHENG Xiaolin,YAN Xingke,YANG Yanping..基于cAMP/PKA通路探讨"调气明目"穴方对单眼形觉剥夺大鼠视觉可塑性的调节机制[J].针灸推拿医学（英文版）,2025,23(6):476-484,9.基金项目
This work was supported by the Projects of National Natural Science Foundation of China(国家自然科学基金项目,No.82260965,No.81260560) （国家自然科学基金项目,No.82260965,No.81260560）
Project of Natural Science Foundation of Gansu Province(甘肃省自然科学基金项目,No.22JR5RA583) （甘肃省自然科学基金项目,No.22JR5RA583）
Special Fund for"Qi Huang Elite Talent"Supervisor of Gansu University of Chinese Medicine(甘肃中医药大学"岐黄英才"导师专项基金,No.ZYXKSD-202220,No.ZYXKBD-202208). （甘肃中医药大学"岐黄英才"导师专项基金,No.ZYXKSD-202220,No.ZYXKBD-202208）
