安徽农业科学2025，Vol.53Issue(24)：32-35,4.DOI:10.3969/j.issn.0517-6611.2025.24.006
基于响应面法的"十色宝"野生彩茶高效增殖条件筛选与优化
Response Surface Methodology for Optimaization of Proliferation Cultures of"shisebao"Camellia sinensis
摘要
Abstract
In order to obtain the best way of tissue culture proliferation of"shisebao"Camellia sinensis,the axillary buds of"shisebao"Ca-mellia sinensis were used as explants.The effects of basic medium,6-BA,IBA and GA3 on the proliferation of"shisebao"Camellia sinensis were studied by single factor experiment and response surface methodology.The results showed that B5+1.07 mg/L 6-BA+0.47 mg/L IBA+2.08 mg/L GA3 was the best proliferation medium,and the proliferation rate was 4.27.Under this condition,the stem of"shisebao"Camellia sinensis was thick with dark green leaves,and the repeatability was good.关键词
野生彩茶"十色宝"/组织培养/继代增殖/有效芽Key words
"shisebao"Camellia sinensis/Tissue culture/Subculture multiplication/Effective bud分类
农业科技引用本文复制引用
刘天天,陈雪洋,石战,贾晓宇,杨迪..基于响应面法的"十色宝"野生彩茶高效增殖条件筛选与优化[J].安徽农业科学,2025,53(24):32-35,4.基金项目
河南省高等学校重点科研项目计划支持(20B210014). （20B210014）
