安徽医科大学学报2025，Vol.60Issue(11)：1996-2004,9.DOI:10.19405/j.cnki.issn1000-1492.2025.11.002
等离子体活化水协同白及多糖制备水凝胶的抗菌性能及相容性
Antibacterial properties and compatibility of plasma-activated water/bletilla striata polysaccharide composite hydrogel
摘要
Abstract
Objective To investigate the antibacterial properties and biocompatibility of a novel hydrogel fabricated by integrating plasma-activated water(PAW)and bletilla striata polysaccharide(BSP).Methods The experi-ments were carried out by dividing the hydrogels into four groups based on their compositions:the deionized water(DW)-carbomer 940(CBM940)-carboxymethyl chitosan(CMCS)hydrogel group(H group),the PAW-CBM940-CMCS hydrogel(PAH)group,the DW-BSP-CBM940-CMCS hydrogel(BSPH)group,and the PAW-BSP-CBM940-CMCS hydrogel(PA/BSPH)group.Physical properties of the hydrogels were evaluated by testing water loss rate and water vapor transmission rate.The content of active substances was determined using a microplate reader and an electron spin resonance spectrometer(ESR).The pH and ORP values were measured using a pH meter and an oxidation-reduction potential(ORP)electrode.The antibacterial mechanism was elucidated by analyzing the integ-rity of bacterial biofilms.Antibacterial activity was evaluated via the zone of inhibition assay.Cytotoxicity testing was performed using the cell counting kit-8(CCK-8)assay,and combined with the cell scratch assay,to collec-tively evaluate the effects of the hydrogel on cellular biocompatibility and migration ability.Results The water loss rate and water vapor transmission rate of PA/BSPH hydrogel were(32.3±2.3)％and(2 228.2±181.1)g/(m2·d).Compared with the H group hydrogel,the contents of hydrogen peroxide(H2O2)and hydroxyl radicals(·OH)in PAH and PA/BSPH groups significantly increased(P＜0.05),and the diameters of inhibition zones significantly enlarged(P＜0.05).The cell viability in PAH group significantly decreased(P＜0.05).The PA/BSPH group showed non-toxicity and a higher cell migration rate(P＜0.05).Conclusion The antibacterial mechanism of the PA/BSPH hydrogel relies on the reactive species H2O2 and·OH in PAW.The incorporation of BSP enhances the water retention and breathability of the wound dressing while significantly reducing the cytotoxici-ty of PAW.This modification endows the hydrogel with improved biocompatibility and promotes cell proliferation.The PA/BSPH hydrogel demonstrates clinical potential by offering a novel therapeutic strategy for chronic infected wound management.关键词
等离子体活化水/白及多糖/水凝胶/伤口愈合/抗菌/细胞毒性Key words
plasma-activated water/bletilla striata polysaccharide/hydrogel/wound healing/antimicrobial/cyto-toxicity分类
医药卫生引用本文复制引用
任文雪,程诚,韩伟,刘涛峰..等离子体活化水协同白及多糖制备水凝胶的抗菌性能及相容性[J].安徽医科大学学报,2025,60(11):1996-2004,9.基金项目
国家自然科学基金(编号:U20A20372) National Natural Science Foundation of China(No.U20A20372) （编号:U20A20372）
