安徽医科大学学报2025，Vol.60Issue(11)：2005-2010,6.DOI:10.19405/j.cnki.issn1000-1492.2025.11.003
TRIM21与顺铂诱导小鼠急性肾损伤的相关性
Correlation between TRIM21 and Cisplatin induced acute kidney injury in mice
摘要
Abstract
Objective To investigate the expression of triple motif protein 21(TRIM21)at different time points in Cisplatin induced acute kidney injury(Cis-AKI)mouse model and its correlation with pathological score.Methods Acute kidney injury(AKI)model was induced by intraperitoneal injection of cisplatin(20 mg/kg).Peripheral blood serum was collected at 12,24,48 and 72 h,respectively.Body weight and kidney mass were also recorded.The dynamic changes of serum creatinine(SCr)and urea nitrogen(BUN)were detected.The expressions of TRIM21 protein and endoplasmic reticulum stress(ERS)proteins GRP78,GRP94,P-elf2α and CHOP were de-tected by Western blot.HE staining was used to observe the pathology of renal tissue and analyze the correlation between the expression of TRIM21 protein at different time points.Results Compared with the control group,the renal body ratio of Cis-AKI mice significantly increased at 48 h and 72 h(P＜0.01).SCr and BUN of Cis-AKI mice significantly increased at 48 h and 72 h compared with control group(P＜0.01).HE staining showed that compared with the control group,the renal pathological scores of Cis-AKI mice increased at 12 and 24 h(P＜0.000 1).At the same time,the expression of TRIM21 protein significantly increased at 24 h(P＜0.01).Com-pared with the control group,the renal pathological score of Cis-AKI mice significantly increased at 48 h(P＜0.000 1),and the protein expression of TRIM21,CHOP and GRP78 was significantly up-regulated at 48 h(P＜0.05).Compared with the control group,the renal pathological score of Cis-AKI mice significantly increased at 72 h(P＜0.000 1),and the expression of TRIM21,GRP94 and P-elf2α proteins was significantly up-regulated at 72 h(P＜0.05).The results of correlation analysis showed that the expression of TRIM21 protein at different time points was positively correlated with the HE pathological scores at different time points(P＜0.000 1).Conclusion At different time points,TRIM21 may participate in the pathological progression of Cis-AKI by affecting ERS,and it is positively correlated with renal pathological scores,which is expected to become a potential early diagnos-tic marker and intervention target.关键词
急性肾损伤/顺铂/TRIM21/不同时间点/内质网应激/肾脏病理评分Key words
acute kidney injury/cisplatin/TRIM21/different time points/endoplasmic reticulum stress/renal pathological score分类
医药卫生引用本文复制引用
孙晴晴,倪蕾,魏伟,王春..TRIM21与顺铂诱导小鼠急性肾损伤的相关性[J].安徽医科大学学报,2025,60(11):2005-2010,6.基金项目
安徽省重点研究与开发计划(编号:2023s07020003) Key Research and Development Program of Anhui Province(No.2023s07020003) （编号:2023s07020003）
