南方农业学报2025，Vol.56Issue(10)：3284-3294,11.DOI:10.3969/j.issn.2095-1191.2025.10.026
水体Cu2+对罗氏沼虾溞状幼体生长发育、存活及抗氧化功能的影响
Effects of waterborne Cu2+on the growth,development,survival,and antioxidant function of Macrobrachium rosenbergii zoea larvae
摘要
Abstract
[Objective]This study aimed to investigate effects of waterborne Cu2+on the growth,development,sur-vival,and antioxidant function of zoea larvae of Macrobrachium rosenbergii,so as to provide a theoretical reference for water quality safety management in the process of Macrobrachium rosenbergii aquaculture.[Method]Using healthy Macrobrachium rosenbergii zoea larvae at stage Ⅰ as tested subjects,the experiment set a control group(Cu2+≤0.001 mg/L)and six Cu2+concentration gradient treatment groups(0.0025,0.0100,0.0500,0.1000,0.1500,and 0.2000 mg/L),with the culture period of 25 d.The developmental stages of zoea larvae in each group was observed every 5 d using a micro-scope,and their body length and survival rate were recorded.The activities of copper-zinc superoxide dismutase(Cu/Zn-SOD),catalase(CAT),glutathione peroxidase(GPx),and malondialdehyde(MDA)content were detected using kits.Total RNA was extracted,and the relative expressions of the antioxidant genes Cu/Zn-SOD,CAT,and GPx were de-tected.[Result]As Cu2+concentration increased,the daily body length growth rate and emergence rate of zoea larvae of Macrobrachium rosenbergii showed a trend of first increasing and then decreasing.In the 0.1000 mg/L group,the zoea larvae exhibited the highest daily body length growth rate and emergence rate,and their survival rate was higher than that in the 0.1500 mg/L and 0.2000 mg/L groups at all sampling time.Zoea larvae in the 0.0500 mg/L and 0.1000 mg/L groups underwent faster molting and metamorphosis;in the 0.1500 mg/L and 0.2000 mg/L groups,zoea larvae experienced inhibited development;in the 0.2000 mg/L group,zoea larvae could not fully metamorphose into shrimp larvae.The MDA contents in the control group,0.0025 mg/L,0.0100 mg/L,0.0500 mg/L,and 0.1000 mg/L groups generally showed a trend of first increasing and then decreasing,with no significant differences among the groups treated with Cu2+for the same time(P＞0.05).At the same Cu2+treatment time,the relative expressions of Cu/Zn-SOD,CAT,and GPx genes generally showed a trend of first increasing and then decreasing as the Cu2+concentration increased:on 5 d,10 d,and 15 d,the relative expressions of Cu/Zn-SOD,CAT,and GPx genes in the 0.1000 mg/L group were higher than those in other groups;on 20 d and 25 d,the relative expressions of Cu/Zn-SOD,CAT,and GPx genes in the 0.1500 mg/L and 0.2000 mg/L groups were significantly lower than those in other groups(P＜0.05).[Conclusion]The growth,develop-ment,survival,and antioxidant function of zoea larvae of Macrobrachium rosenbergii exhibit an obvious concentration-dependent effect on Cu2+concentration.When Cu2+≤0.1000 mg/L,it can induce a moderate antioxidant stress response,thus promoting the growth and development of zoea larvae of Macrobrachium rosenbergii and improving their survival rate.When Cu2+≥0.1500 mg/L,the domination of Cu2+pro-oxidative effect of leads to a large(reactive oxygen species)ROS accumulation that exceeds the body's clearance capacity,causing severe oxidative damage,thereby ultimately causing growth inhibition and causing developmental stagnation of zoea larvae of Macrobrachium rosenbergii.关键词
Cu2+/罗氏沼虾/溞状幼体/变态发育/存活率/抗氧化功能Key words
Cu2+/Macrobrachium rosenbergii/zoea larvae/metamorphosis/survival rate/antioxidant function分类
农业科技引用本文复制引用
章轲,戴习林..水体Cu2+对罗氏沼虾溞状幼体生长发育、存活及抗氧化功能的影响[J].南方农业学报,2025,56(10):3284-3294,11.基金项目
上海市科技兴农重点攻关项目(G2021-02-08-00-12-F00748) Shanghai Agriculture Applied Technology Development Program(G2021-02-08-00-12-F00748) （G2021-02-08-00-12-F00748）
