南方农业学报2025，Vol.56Issue(10)：3295-3306,12.DOI:10.3969/j.issn.2095-1191.2025.10.027
绵羊源马赛阿克曼氏菌分离培养及其功能分析
Isolation,cultivation,and function analysis of sheep-derived Akkermansia massiliensis
摘要
Abstract
[Objective]This study aimed to perform isolation,culture,and functional analysis of sheep-derived Akker-mansia massiliensis,so as to provide a theoretical basis for the control of intestinal inflammatory diseases of sheep and the probiotic potential of sheep-derived Akkermansia massiliensis in intestinal health.[Method]Fresh sheep feces were collected to isolate and identify an Akkermansia massiliensis strain under the anaerobic condition at 37 ℃.The genomic characteristics and potential functions were analyzed by whole-genome sequencing.A mouse model of colitis induced by sodium dextran sulfate(DSS)was constructed,and the inhibitory effects of Akkermansia massiliensis on intestinal inflammation and its regulatory role in the intestinal microbiota composition were evaluated via gavage intervention.[Result]Ms2526 strain showed a 100%genome integrity,a contamination rate of 0.12%,a coding density of 0.874,a genome length of 3112518 bp,a GC content of 58%,and a total of 2622 genes.Ms2526 was most closely related to the EB-AMDK-40 of Akkermansia massiliensis,clustering together with a 99%self-spreading support rate,and differed in branch length from other Akkermansia massiliensis strains such as Akk2000.The Ms2526 genome contained coding genes highly homologous to Amuc_1100 and P9 proteins.Coding genes of Ms2526 genome were mostly enriched in metabolic pathways,including signaling pathways related to carbohydrate metabolism,cofactor and vitamin metabolism,biosyn-thesis of other secondary metabolites,and terpenes and polyketides metabolism.Five genes were enriched in immune sys-tem signaling pathways among the organismal system categories.The Ms2526 genome contained 157 genes annotated to six major classes of carbo-hydrate active enzymes,with higher numbers of genes annotated to glycosyltransferases and glycoside hydrolases.The Ms2526 genome contained one macrolide antibiotic resistance gene,erm(B).The Ms2526 could alleviate DSS-induced colitis in mice,improve the integrity of colonic tissues and mucosal structure,and increase the abundance of intestinal microbiota species.[Conclusion]The Ms2526 genome of sheep-derived Akkermansia massilien-sis has genetic basis related to carbohydrate metabolism,immune homeostasis regulation,and mucosal repair.The Ms2526 strain effectively alleviates DSS-induced colitis in mice,and its effect is closely related to enhancing intestinal barrier integrity,regulating systemic inflammation,and restoring the richness of intestinal microbiota.The Ms2526 strain demonstrates good probiotic potential in regulating intestinal health.关键词
马赛阿克曼氏菌/全基因组序列分析/结肠炎/肠道菌群Key words
Akkermansia massiliensis/whole-genome sequence analysis/colitis/intestinal microbiota分类
农业科技引用本文复制引用
葛文静,胡圣伟,戴继鸿,吕园梦,胥欣瑶..绵羊源马赛阿克曼氏菌分离培养及其功能分析[J].南方农业学报,2025,56(10):3295-3306,12.基金项目
国家科技重大专项(2021xjkk0605) （2021xjkk0605）
兵团重点领域科技攻关计划项目(2024AB050) National Major Science and Technology Program(2021xjkk0605) （2024AB050）
Science and Technology Program in Priority Fields of Xinjiang Production and Construction Corps(2024AB050) （2024AB050）
