广州化学2025，Vol.50Issue(6)：82-86,5.DOI:10.16560/j.cnki.gzhx.20252703
正交试验法优化微波辅助提取蛤蜊中无机砷
Optimization of Microwave-assisted Extraction of Inorganic Arsenic in Clam by Orthogonal Test Methodology
孙家豪 1程静秋 1颜可涵 1连旭钟 1林剑豪1
作者信息
- 1. 中科检测技术服务(广州)股份有限公司,广东 广州 510650
- 折叠
摘要
Abstract
The microwave-assisted extraction conditions were optimized through orthogonal experiments.This study used the national certified reference material(clam quality control sample)as raw material.A three-factor three-level orthogonal experimental design L9(33)was employed to determine inorganic arsenic extraction conditions,combined with liquid chromatography-atomic fluorescence spectrometry(LC-AFS)for inorganic arsenic quantification.The orthogonal experiment results revealed that the optimal extraction conditions were:nitric acid volume 14mL,nitric acid concentration 0.12 mol/L,extraction temperature110℃,and extraction time 25 minutes.The theoretical inorganic arsenic content in clam was 0.62 mg/kg,while the measured content reached 0.60 mg/kg with a relative deviation of 2.4%.The standard recovery rate reached 92.0%～95.0%,demonstrating the accuracy and reliability of this orthogonal experimental model.Compared with traditional hot leaching methods,this approach offers advantages such as shorter processing time,lower solvent usage,and operational convenience and rapidity.This study provides scientific basis for clam detection research and quality safety control of aquatic products.关键词
正交试验法/微波辅助提取/蛤蜊/无机砷/液相原子荧光Key words
orthogonal test methodology/microwave-assisted extraction/clam/inorganic arsenic/LC-AFS分类
轻工纺织引用本文复制引用
孙家豪,程静秋,颜可涵,连旭钟,林剑豪..正交试验法优化微波辅助提取蛤蜊中无机砷[J].广州化学,2025,50(6):82-86,5.
