湖北农业科学2025，Vol.64Issue(11)：80-85,6.DOI:10.14088/j.cnki.issn0439-8114.2025.11.010
广西姜黄炭疽病病原鉴定及生物学特性分析
Identification and biological characteristics analysis of the pathogen causing anthracnose on Curcuma longa in Guangxi
摘要
Abstract
To identify the pathogenic species responsible for anthracnose on Curcuma longa in Guangxi and characterize its biological properties,the tissue isolation method was adopted for pathogen isolation.Pathogens were identified through pathogenicity verifica-tion,morphological characterization,and multigene phylogenetic analysis incorporating ITS,ACT,and GAPDH sequences,while their biological characteristics were simultaneously determined.The results showed that 47 fungal isolates were purified from diseased Curcuma longa plants.According to colony morphology and microscopic morphology,36 isolates with the highest isolation frequency and consistent morphology were classified into the same group,and the representative strain JH44-8 was selected for subsequent stud-ies.Integrated morphological assessment and multigene phylogenetic analysis confirmed that the pathogen was Colletotrichum curcum-ae.Studies on biological characteristics showed that the mycelia of this strain grew best under the following conditions:temperature of 25-28℃,pH of 7-9,dark environment,and on PSA,SDA,and PDA media;the optimal carbon source was maltose and the optimal nitrogen source was peptone;the lethal condition for its mycelia was treatment at 55℃for 20 minutes.关键词
姜黄(Curcuma longa L.)/炭疽病/病原鉴定/姜黄炭疽菌(Colletotrichum curcumae)/生物学特性Key words
Curcuma longa L./anthracnose/pathogen identification/Colletotrichum curcumae/biological characteristics分类
农业科技引用本文复制引用
詹鑫婕,梁文静,黄媛,杨东昱,蒋妮,黄琦,丘卓秋,韦树根,白丹宇,宋利沙,李玉琼..广西姜黄炭疽病病原鉴定及生物学特性分析[J].湖北农业科学,2025,64(11):80-85,6.基金项目
广西中医药适宜技术开发与推广项目(GZSY23-01) （GZSY23-01）
广西重点研发计划项目(桂科AB25069170) （桂科AB25069170）
广西中医药多学科交叉创新团队项目(GZKJ2305) （GZKJ2305）
广西壮族自治区中医药管理局项目(GXZYA20230006 （）
GXZYA20230012) （）
