甘蔗糖业2025，Vol.54Issue(6)：71-77,7.DOI:10.3969/j.issn.1005-9695.2025.06.009
纤维素气凝胶@ZIF-8制备及在纤维素酶固定化中应用
Preparation of Cellulose Aerogel@ZIF-8 and Its Application in Cellulase Immobilization
摘要
Abstract
Cellulose aerogel,as an environmentally friendly and multifunctional material,was utilized in this study to develop an efficient composite carrier for cellulase immobilization.Using microcrystalline cellulose as the raw material,cellulose aerogel was fabricated through processes including ultrasonic dispersion,cross-linking,and freeze-drying.Subsequently,a cellulose aerogel@ZIF-8 composite with a high specific surface area was constructed via the coordination of zinc sulfate and 2-methylimidazole in an aqueous phase,forming a metal-organic framework(MOF)based material for cellulase immobilization.The preparation conditions of the aerogel and the parameters for cellulase immobilization were optimized through single-factor experiments and orthogonal tests.The optimal immobilization conditions were determined as follows:glutaraldehyde dosage of 0.5 mL,cross-linking time of 4 h,and ultrasonic time of 25 min.Under these conditions,the immobilized cellulase activity reached 86.135 U/mg.This study provides a novel strategy for the efficient recovery and reuse of cellulase,demonstrating significant theoretical and practical value.关键词
纤维素气凝胶/ZIF-8/纤维素酶/固定化Key words
Cellulose aerogel/ZIF-8/Cellulase/Immobilization分类
轻工纺织引用本文复制引用
梁玉燕,谭涵钰,翁绮纹,尚红岩,李泳恒,陈铭嘉,姚采儿,陈柳婷,黄璟琦,陈可欣,肖虹..纤维素气凝胶@ZIF-8制备及在纤维素酶固定化中应用[J].甘蔗糖业,2025,54(6):71-77,7.基金项目
广东轻工职业技术大学大学生科研项目(XSKYL202432) （XSKYL202432）
广东轻工职业技术大学创新创业精致育人项目(JZYR202404) （JZYR202404）
广东轻工职业技术大学第二十五届"挑战杯" （）
广州市海珠区科技计划项目(海科工商信计2022-41) （海科工商信计2022-41）
广东轻工职业技术大学校级科研项目("挑战杯"竞赛专项)(tzb04A06) （"挑战杯"竞赛专项）
