高原农业2025，Vol.9Issue(6)：739-747,9.DOI:10.19707/j.cnki.jpa.2025.06.007
西藏野生宽叶韭花粉离体萌发特性研究
A Study of the In Vitro Germination Characteristics of Wild Allium hookeri Pollen in Xizang
摘要
Abstract
To investigate the in vitro germination characteristics of wild Allium hookeri pollen,this study focused on Xizang A.hookeri pollen.First,single-factor experiments were conducted to preliminarily identify optimal light conditions,temperature,incubation time,and suitable concentration ranges of sucrose,boric acid,and calcium chloride in the culture medium for pollen germination.Subsequently,orthogonal experiments were employed to evaluate the interactive effects of these components on pollen germination and to screen for the optimal medium formulation.The results showed that dark conditions were more conducive to pollen germination,the optimal germination temperature was 25℃,and the optimal culture time was 1 h.The results of single factor test showed that sucrose concentration was 100～150 g/L,boric acid concentration was 100～150 mg/L and calcium chloride concentration was 0～50 mg/L in the suitable medium for A.hookeri pollen germination.In the orthogonal test,the highest pollen germination rate was 57.39%under sucrose 100 g/L,boric acid 100 mg/L and calcium chloride 0 mg/L,and the lowest pollen germination rate was 34.26%under sucrose 100 g/L,boric acid 200 mg/L and calcium chloride 150 mg/L.The pollen tube under the conditions of sucrose 50 g/L,boric acid 100 mg/L and calcium chloride 0 mg/L was the longest(40.76 μm),while the pollen tube under the conditions of sucrose 150 g/L,boric acid 200 mg/L and calcium chloride 150 mg/L was the shortest(19.11 μm).Platyphylla,while high concentration of calcium chloride could inhibit it.In conclusion,the optimal culture conditions for in vitro germination of A.hookeri pollen were dark culture at 25℃for 1 h,and the optimal medium combination was sucrose 100 g/L+boric acid 100 mg/L+calcium chloride 0 mg/L.关键词
宽叶韭/花粉/离体萌发/培养基Key words
Allium hookeri/Pollen/Germination in vitro/Culture medium分类
农业科技引用本文复制引用
段双双,徐湖,张子骏,马晓帆,王忠红..西藏野生宽叶韭花粉离体萌发特性研究[J].高原农业,2025,9(6):739-747,9.基金项目
国家自然科学基金项目(32360758) （32360758）
西藏自治区自然科学基金项目(XZ202001ZR0013G). （XZ202001ZR0013G）
