河北医学2025，Vol.31Issue(12)：1943-1949,7.DOI:10.3969/j.issn.1006-6233.2025.12.02
地佐辛调节p38MAPK/JNK信号通路对子宫内膜癌细胞增殖侵袭凋亡等影响
Effect of Dezocine on the Proliferation Invasion and Apoptosis of Endometrial Carcinoma Cells via Regulating p38MAPK/JNK Signaling Pathway
摘要
Abstract
Objective:To explore the effects of dezocine(Dez)on the proliferation,apoptosis,and epi-thelial-mesenchymal transition of uterine corpus endometrial cancer(UCEC)cells by regulating p38 mitogen-activated protein kinase 38(p38MAPK)/c-Jun N-terminal kinase(JNK)signaling pathway.Methods:Human UCEC cells HEC-1A were cultured in vitro and randomly assigned into NC group,low-dose Dez group,medium-dose Dez group,high-dose Dez group(2.5,5,10μg/mL Dez),and high-dose Dez+SB203580 group(10μg/mL Dez+10μmol/L p38MAPK inhibitor SB203580).MTS method was used to detect the proliferation of HEC-1A cells.The plate cloning method was used to measure the clonogenic ability of HEC-1A cells.Flow cytometry was used to measure apoptosis of HEC-1A cells.Transwell experiment was used to detect the invasion of HEC-1A cells.Western blot was used to measure the E-cadherin,N-cadherin,Snail,and p38MAPK/JNK signaling pathway proteins.Results:For the NC group,the low-dose,medium-dose,and high-dose Dez groups showed a decrease in D(λ)490nm,colony formation rate,cell invasion num-ber,N-cadherin,and Snail proteins in HEC-1A cells,and an increase in apoptosis rate,E-cadherin,p-p38MAPK/p38MAPK,and p-JNK/JNK proteins(P<0.05).For the high-dose Dez group,the high-dose Dez+SB203580 group showed an increase in D(λ)490nm,colony formation rate,cell invasion number,N-cadherin,and Snail proteins in HEC-1A cells,and a decrease in apoptosis rate,E-cadherin,p-p38MAPK/p38MAPK,and p-JNK/JNK proteins(P<0.05).Conclusion:Dez exerts inhibitory effects on the prolifera-tion,invasion,and epithelial mesenchymal transition of UCEC cells,while promoting UCEC cells apoptosis.This is likely mediated through activating the p38MAPK/JNK signaling pathway.关键词
子宫内膜癌/地佐辛/p38丝裂原活化蛋白激酶38/c-Jun氨基末端激酶/细胞增殖/细胞凋亡/上皮间质转化Key words
Endometrial cancer/Dezocine/P38 mitogen activated protein kinase 38/c-Jun N-terminal kinase/Cell proliferation/Cell apoptosis/Epithelial mesenchymal transition引用本文复制引用
李传进,曹晓霞,丁章俊..地佐辛调节p38MAPK/JNK信号通路对子宫内膜癌细胞增殖侵袭凋亡等影响[J].河北医学,2025,31(12):1943-1949,7.基金项目
湖北省卫生健康委科研项目,(编号:WJ2023M117) （编号:WJ2023M117）
