河北医学2025，Vol.31Issue(12)：1955-1963,9.DOI:10.3969/j.issn.1006-6233.2025.12.04
miR-93-5p调控TXNIP/NLRP3通路对脑出血大鼠神经功能损伤的影响
Effect of miR-93-5p on Neurological Dysfunction in Rats with Cerebral Hemorrhage by Adjusting TXNIP/NLRP3 Pathway
摘要
Abstract
Objective:To investigate the impacts of miR-93-5p on neurological dysfunction and thiore-doxin-interacting protein/nucleotide-binding oligomerization domain-like receptor protein 3(TXNIP/NLRP3)pathway in rats with cerebral hemorrhage(ICH).Methods:An ICH rat model was constructed,and successfully modeled rats were randomly grouped into model group(ICH group),miR-NC group,miR-93-5p mimics group,miR-93-5p mimics+pcDNA3.1 group,and miR-93-5p mimics+pcDNA-TXNIP group,each with 18 rats.Additionally,18 normal healthy rats served as control group.Longa rating was con-ducted.ELISA was used to test serum inflammatory factors.HE staining was used to observe the pathological morphology of brain tissue.TUNEL staining was performed to test neuronal apoptosis.Immunohistochemistry was performed to test Iba1.Western blot was used to detect TXNIP/NLRP3 pathway related proteins.The du-al luciferase assay report was used to test the targeting relationship between miR-93-5p and TXNIP.Results:For control group,ICH group revealed clear structural damages to brain tissue,degeneration and necrosis of cells,loose and disordered arrangement,nuclear shrinkage and deep staining,conspicuous interstitial edema,and inflammatory cell infiltration,and the Longa score,brain tissue water content,IL-1β,IL-18,apoptosis rate,Bax,C-caspase-9,C-caspase-3,Iba1,TXNIP,NLRP3,ASC,and C-caspase-1 elevated(P<0.05).The miR-93-5p mimics group showed less pathological damage to brain tissue than the miR-NC group,and the Longa score,brain tissue water content,IL-1β,IL-18,apoptosis rate,Bax,C-caspase-9,C-caspase-3,Iba1,TXNIP,NLRP3,ASC,and C-caspase-1 reduced(P<0.05).The miR-93-5p mimics+pcDNA3.1 group showed more severe brain tissue pathological damage than the miR-93-5p mimics+pcDNA-TXNIP group,and the Longa score,brain tissue water content,IL-1β,IL-18,apoptosis rate,Bax,C-caspase-9,C-caspase-3,Iba1,TXNIP,NLRP3,ASC,and C-caspase-1 elevated(P<0.05).In addi-tion,Starbase website predictions and dual luciferase assay reports indicated a targeted relationship between miR-93-5p and TXNIP.Conclusion:MiR-93-5p can exert a protective effect on neurological damage in ICH rats,which is related to the inhibition of TXNIP/NLRP3 pathway.关键词
脑出血/miR-93-5p/TXNIP/NLRP3通路/神经功能损伤Key words
Intracerebral hemorrhage/miR-93-5p/TXNIP/NLRP3 pathway/Neurological dysfunction引用本文复制引用
安世恩,赵欢,周涛,高鹏竹,常祥平,高建洲..miR-93-5p调控TXNIP/NLRP3通路对脑出血大鼠神经功能损伤的影响[J].河北医学,2025,31(12):1955-1963,9.基金项目
河北省卫生健康委员会办公室年度医学科学研究课题,(编号:20240383) （编号:20240383）
