河北医学2025，Vol.31Issue(12)：1963-1969,7.DOI:10.3969/j.issn.1006-6233.2025.12.05
黄腐酚调节CaMKK2/AMPK信号通路对急性脑梗死大鼠神经元凋亡及炎症反应的影响
Effects of Xanthohumol on Neuronal Apoptosis and Inflammatory Response in Rats with Acute Cerebral Infarction by Regulating CaMKK2/AMPK Signaling Pathway
摘要
Abstract
Objective:To explore the effects of xanthohumol on neuronal apoptosis and inflammatory re-sponse in rats with acute cerebral infarction by regulating calcium/calmodulin dependent protein kinase 2(CaMKK2)/AMP-activated protein kinase(AMPK)signaling pathway.Methods:In this study,an acute cerebral infarction model was established by occlusion of the middle cerebral artery,and was assigned into the cerebral infarction group,the low-dose(L)xanthohumol group(25mg/kg),the high-dose(H)xanthohu-mol group(50mg/kg),and the H-xanthohumol+inhibitor group,with 12 rats in each.The medication was administered for 28days.Another 12 healthy rats were made the normal group.Longa scoring method was per-formed to evaluate the neurological function of rats.TTC staining was performed to measure the volume of cer-ebral infarction.HE and TUNEL staining were used to observe brain tissue lesions and cell apoptosis in rats.ELISA was used to detect the inflammatory factors interleukin(IL)-1β,tumor necrosis factor-α(TNF-α),and IL-6 in rat brain homogenates.Western blot was performed to detect the CaMKK2 and AMPK related pro-teins.Results:For the normal group,the cerebral infarction group showed improved neurological function scores,cerebral infarction,increased cell apoptosis rate,IL-1β,TNF-α,IL-6,and decreased p-CaMKK2 and p-AMPK(P<0.05).For the cerebral infarction group,the L-xanthohumol group and H-xanthohumol group showed decreased neurological function scores,reduced cerebral infarction volume,reduced brain tissue damage,decreased cell apoptosis rate,IL-1β,TNF-α,IL-6,and increased p-CaMKK2 and p-AMPK(P<0.05),and the therapeutic effect of H-xanthohumol was better than that of L-xanthohumol(P<0.05).The CaMKK2 inhibitor STO-609 eliminated the inhibitory effects of xanthohumol on neuronal apoptosis and inflam-matory response in rats with acute cerebral infarction(P<0.05).Conclusion:Xanthohumol may alleviate neuronal apoptosis and inflammatory response in rats with acute cerebral infarction by activating CaMKK2/AMPK signaling pathway.关键词
急性脑梗死/黄腐酚/神经元凋亡/CaMKK2/AMPK信号通路Key words
Acute cerebral infarction/Xanthohumol/Neuronal apoptosis/CaMKK2/AMPK signaling pathway引用本文复制引用
常亮,李明,李晶,刘照寒..黄腐酚调节CaMKK2/AMPK信号通路对急性脑梗死大鼠神经元凋亡及炎症反应的影响[J].河北医学,2025,31(12):1963-1969,7.基金项目
黑龙江省卫生健康委员会科研项目,(编号:2020021) （编号:2020021）
