河北医学2025，Vol.31Issue(12)：1969-1976,8.DOI:10.3969/j.issn.1006-6233.2025.12.06
益气化瘀方调节TLR4/NF-κB通路介导细胞焦亡改善大鼠子宫内膜炎
Regulation of TLR4/NF-κB Pathway by Yiqi Huayu Formula Ameliorates Endometritis in Rats by Mediating Cellular Focal Death
摘要
Abstract
Objective:To explore the regulation of the Toll-like receptor 4/nuclear factor κB(TLR 4/NF-κ B)pathway-mediated cell pyroptosis to improve endometritis in rats.Methods:Fifty-five SPF-grade rats were selected as the experimental subjects and divided into five groups of 11 rats each by random number table method,which were grouped into 11 blank groups,model group,traditional Chinese medicine control group,Yiqi Huayuquan group,and Yiqi Huayuquan+TAK242 group,each with 10 rats,after being processed by endometritis modeling.The levels of inflammatory factors,oxidative stress,pathological scores and relative expressions of TLR4 and NF-κB were compared among the rats in each group.Results:Com-pared with the blank group,the model group,the Chinese medicine control group,and the Yiqi Huayuquan+TAK242 group showed increases in pathological composite scores,uterine index,MDA,MPO,IL-8,IL-6,IL-2,IL-1β,Caspase-1,GSDMD levels,IκBα,TLR4,and NF-κBp65 proteins,and decreases in LgM,LgG,IgA,and SOD levels.Compared with the model group,IL-8,IL-6,IL-2,IL-1β levels and patholog-ical composite scores,uterine index,MPO,MDA,Caspase-1,GSDMD levels,IκBα,TLR4,NF-κBp65 protein were decreased,and LgM,LgG,IgA,SOD were elevated in the traditional Chinese medicine control group and Yiqi Huayuquan group.Compared with the group of Yiqi Huayu Fang+TAK242,the pathology composite score,uterine index and the levels of IL-8,IL-6,IL-2,IL-1β,caspase-1,GSDMD,IκBα,TLR4,NF-κBp65,MDA and MPO proteins were increased significantly,and the levels of LgM,LgG,IgA and SOD were decreased significantly,with a statistically significant difference(P<0.05).Conclusion:Yiqi Huayu Fang can exert therapeutic effects by inhibiting the TLR4/NF-κB pathway to attenuate cellular pyropto-sis,regulating the activity of inflammatory factors,and improving oxidative stress and immune function.关键词
益气化瘀方/Toll样受体4/核因子κB通路/细胞焦亡/子宫内膜炎Key words
Yiqi huayu recipe/Toll-like receptor 4/nuclear factor κB pathway/Pyroptosis/Endometritis引用本文复制引用
卢利霞,林晓华,杨丹丹..益气化瘀方调节TLR4/NF-κB通路介导细胞焦亡改善大鼠子宫内膜炎[J].河北医学,2025,31(12):1969-1976,8.基金项目
河北省中医药管理局课题,(编号:2023027) （编号:2023027）
