河北医学2025，Vol.31Issue(12)：1976-1983,8.DOI:10.3969/j.issn.1006-6233.2025.12.07
推拿调节BDNF/Trkb/p38/JNK通路对肌筋膜疼痛综合征大鼠的改善作用研究
Study on the Ameliorative Effect of Massage to Regulate BDNF/Trkb/p38/JNK Pathway on Rats with Myofascial Pain Syndrome
摘要
Abstract
Objective:To investigate the study of the ameliorative effect of nudging to regulate the BD-NF/Trkb/p38/JNK pathway on rats with myofascial pain syndrome.Methods:40 SPF grade rats were selected as experimental subjects and divided into 4 groups of 10 rats each by random number table method,30 were selected to perform modeling of myofascial pain syndrome,a total of 3 failed,and the remaining 27 were grouped into 10 blank groups(normal feeding,no modeling and treatment),9 model groups(normal feeding,modeling,no treatment),9 nudging groups(modeling,normal feeding,and the trigger point localized by 0.5kg of force press),9 nudging+K-252a group(normal rearing,modeling,intervention trigger point local-ized at 0.5kg of force press+500nmol/L concentration of K-252a).After the final intervention in each group,4ml of blood was collected from the abdominal aorta,and spinal cord tissue from the L4-6 lumbar en-largement segment was harvested.Serum inflammatory factors and stress markers in the abdominal aorta blood were detected using ELISA.Expression of the cell marker OX-42 in spinal cord tissue was assessed via immu-nohistochemistry.Western Blot analysis was performed to detect levels of BDNF,Trkb,p38,JNK proteins in the L4-6 lumbar bulging segment spinal cord tissue.Results:Compared with the blank group,the model group,massage group,and massage+K-252a group exhibited statistically significant increases in spontane-ous activity frequency,IL-8,IL-6,MDA,GSH,β-endorphin levels,and OX-42,BDNF,Trkb,p38,and JNK protein expression(P<0.05).SOD,substance P,and IL-10 levels decreased,while thermal withdraw-al latency shortened,with statistically significant differences(P<0.05);Compared with the model group,the massage group exhibited statistically significant reductions in P-substance,MDA,GSH,spontaneous action potential frequency,IL-8,and IL-6 levels,as well as OX-42,BDNF,Trkb,p38,and JNK protein expres-sion levels were significantly lower in the massage group than in the model group(P<0.05).Conversely,lev-els of β-endorphin,SOD,heat-withdrawal latency,and IL-10 were significantly elevated in the massage group(P<0.05);Compared with the massage group,the massage plus K-252a group showed significantly lower levels of SOD,IL-10,β-endorphin,and heat-withdrawal paw latency,with statistically significant differences(P<0.05).Levels of IL-8,spontaneous action potential frequency,IL-6,MDA,GSH,sub-stance P,OX-42 expression,BDNF,Trkb,p38,JNK protein expression levels were significantly increased,with statistically significant differences(P<0.05).Conclusion:Massage therapy can effectively alleviate pain in rat models of myofascial pain syndrome.Its mechanism of action may be related to regulating the BD-NF/Trkb/p38/JNK signaling pathway,inhibiting the expression of OX-42 in the spinal cord,reducing in-flammatory responses,and improving oxidative stress conditions.关键词
推拿/BDNF/Trkb/p38/JNK通路/肌筋膜疼痛综合征Key words
Massage/BDNF/Trkb/p38/JNK pathway/Myofascial pain syndrome引用本文复制引用
吴苏旻,喻仁宇,沈重庆,林丹椿..推拿调节BDNF/Trkb/p38/JNK通路对肌筋膜疼痛综合征大鼠的改善作用研究[J].河北医学,2025,31(12):1976-1983,8.基金项目
国家自然科学基金基础提升计划专项研究课题,(编号:JCZX202419) （编号:JCZX202419）
