河北医学2025，Vol.31Issue(12)：1983-1989,7.DOI:10.3969/j.issn.1006-6233.2025.12.08
针刺结合黄芪建中汤调节PI3K/AKT信号通路对胃溃疡大鼠表皮生长因子胃黏膜功能的影响
Effects of Acupuncture Combined with Huangqi Jianzhong Decoction to Regulate PI3K/AKT Signaling Pathway on Epidermal Growth Factor and Gastric Mucosa Function in Rats with Gastric Ulcer
摘要
Abstract
Objective:To explore the effect of acupuncture combined with Huangqi Jianzhong decoction on improving epidermal growth factor(EGF)and gastric mucosal function in rats with gastric ulcer(GU),and to explore its mechanism based on phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)signa-ling pathway.Methods:GU rat model was established by intragastric glacial acetic acid.Rats were randomly divided into control group(normal rats),model group,Huangqi Jianzhong decoction group(6.8g/kg Huan-gqi Jianzhong decoction by gavage),electroacupuncture group(electroacupuncture stimulating Zusanli and Zhongwan points)and combined group(6.8g/kg Huangqi Jianzhong decoction by gavage and acupuncture stimulating Zusanli and Zhongwan points),with 15 rats in each group.After 2 weeks of treatment,the gastric ulcer area and ulcer index of rats in each group were detected.HE matoxylin eosin(He)staining was used to observe the pathological changes of gastric mucosa.Serum GAStrin(gas),growth hormone inhibin(SS),motilin(MTL),tumor necrosis factor α(TNF-α),interleukin 6(IL-6),prostaglandin E2(PGE2)and epi-dermal growth factor(EGF)were detected by ELISA kit.Western Blot was used to detect the expression level of PI3K/AKT pathway related proteins.Results:Compared with the control group,the model group had gas-tric mucosa ulcer,fewer glands,and a large number of inflammatory cells infiltrated,ulcer area and ulcer in-dex were significantly increased,while,SS,PGE2,EGF,p-PI3K and p-Akt levels were significantly de-creased(P<0.05),and the levels of GAS,MTL,TNF-α and IL-6 were significantly increased(P<0.05).Compared with model group,the gastric mucosal injury of rats in Huangqi Jianzhong Decoction group,acu-puncture group and combined group was significantly improved,the ulcer area and ulcer index were signifi-cantly decreased,SS,PGE2,EGF,p-PI3K and p-Akt levels were significantly increased(P<0.05),and the levels of GAS,MTL,TNF-α and IL-6 were significantly decreased(P<0.05).Compared with Huangqi Jianzhong Decoction group and acupuncture group,the ulcer area and ulcer index were significantly de-creased,SS,PGE2,EGF,p-PI3K and p-Akt levels of rats in combined group were significantly increased·4891·(P<0.05),while the levels of GAS,MTL,TNF-α and IL-6 were significantly decreased(P<0.05).Con-clusion:Huangqi Jianzhong decoction combined with acupuncture can promote the repair and healing of gas-tric mucosa and improve the gastric mucosal function in GU rats by inhibiting inflammatory reaction,and the mechanism involved may be related to the activation of PI3K/AKT signaling pathway.关键词
胃溃疡/黄芪建中汤/电针/PI3K/AKT信号通路/表皮生长因子/胃黏膜功能Key words
Gastric ulcer/Huangqi Jianzhong decoction/Acusector/PI3K/AKT signaling pathway/Epidermal growth factor/Gastric mucosal function引用本文复制引用
武九龙,范钦梅..针刺结合黄芪建中汤调节PI3K/AKT信号通路对胃溃疡大鼠表皮生长因子胃黏膜功能的影响[J].河北医学,2025,31(12):1983-1989,7.基金项目
江苏省优势学科建设工程项目,(编号:YSHL2201-191) （编号:YSHL2201-191）
