农业科技与技术（英文）2025，Vol.26Issue(3)：56-62,7.DOI:10.16175/j.cnki.1009-4229.2025.03.008
基于COI基因的倒刺鲃属鱼类系统分类研究
Phylogenetic classification of Spinibarbus based on COI gene
摘要
Abstract
For the classification of the various species of fishes in the genus Spinibarbus,molecular identification and phylogenetic analysis were conducted for five species of fishes in Spinibarbus based on the mitochondrial COI gene.The results showed that the interspecific genetic distances were greater than 0.02,whereas the intraspecific genetic distances were less than 0.02.The phylogenetic tree revealed that the S.caldwelli and the S.hollandi clustered into clade I.The S.yunnanensis and the S.denticulatus clustered in a branch,and then clustered with the S.sinensis to form clade II.Clades I and II together formed the genus Spinibarbus.The S.caldwelli and S.hollandi clustered in two separate subclades,and the genetic distances between them were greater than the genetic distances between each of them and other species within the same subclade.The results of this study indicated that the mitochondrial COI gene sequences were effective for species identification of fishes in the genus Spinibarbus and could be used to explore the phylogeny of this genus.关键词
倒刺鲃属/物种分类/DNA条形码/COI基因/系统发育Key words
Spinibarbus/Species classification/DNA barcode/COI gene/Phylogeny引用本文复制引用
赖洁,叶树政,黄文炜,李斯迅,邓彬华,韩崇,桂林,李强..基于COI基因的倒刺鲃属鱼类系统分类研究[J].农业科技与技术（英文）,2025,26(3):56-62,7.基金项目
Supported by National Natural Science Foundation of China(42077321) （42077321）
Science and Technology Planning Project of Guangzhou City(201804010486) （201804010486）
College Students'Innovation and Entrepreneurship Project of Guangdong Province in 2024(S202411078013) 国家自然科学基金(42077321) （S202411078013）
广州市科技计划项目(201804010486) （201804010486）
2024年度广东省大学生创新创业项目(S202411078013) （S202411078013）
