河北医学2025，Vol.31Issue(12)：1998-2004,7.DOI:10.3969/j.issn.1006-6233.2025.12.010
电针灸通过mTOR/NLRP3介导的自噬改善卒中后认知功能障碍的研究
Study on Electroacupuncture Moxibustion Improving Post-Stroke Cognitive Dysfunction through mTOR/NLRP3 Mediated Autophagy
摘要
Abstract
Objective:To explore the therapeutic effect and mechanism of electroacupuncture(EA)on cognitive dysfunction in stroke rats.Methods:Totally 42 SD rats were randomly divided into Sham group,MCAO group,MCAO+RAPA group,MCAO+EA group,MCAO+RAPA+EA group,MCAO+EA+Vector group,and MCAO+EA+OE-mTOR group,with 6 rats in each group.The middle cerebral artery embolization(MCAO)model was established,except for the Sham group.Rats in MCAO+RAPA group were intraperitone-ally injected with 3 mg/kg RAPA,rats in MCAO+EA group were treated with electroacupuncture at Fengchi,Fengfu and Dashui points,and rats in MCAO+EA+Vector group and MCAO+EA+OE-mTOR group were in-jected with mTOR overexpression empty vector or mTOR overexpression vector in the lateral ventricle of the brain.The Zea-longa neurological deficit scoring system was used to evaluate neurobehavior.Morris water maze test was used to evaluate cognitive function.2,3,5-triphenyltetrazolium chloride(TTC)staining was used to evaluate the cerebral infarction area.Hematoxylin eosin(HE)staining was used to evaluate the patho-logical changes.Enzyme-linked immunosorbent assay was used to detect the contents of tumor necrosis factor alpha(TNF-α),interleukin-6(IL-6),and interleukin-10(IL-10).Lens electron microscope was used to detect autophagosomes.Western blot was used to detect mTOR,IL-6,IL-10,NLRP3,Beclin-1,p62,LC3 Ⅰ/Ⅱ protein expression.Results:Compared with the Sham group,the Zea longa score,cerebral infarc-tion area,TNF-α and IL-6 level,autophagosome number,Beclin-1,LC3 Ⅰ/Ⅱ,and NLRP3 protein levels in MCAO group increased,while the neurological function score,IL-10 content,p62 and mTOR protein lev-els decreased(P<0.05).Compared with MCAO group,the Zea-longa score,cerebral infarction area,TNF-α and IL-6 level,autophagosome number,Beclin-1,LC3 Ⅰ/Ⅱ and NLRP3 protein levels in MCAO+EA group decreased,while the neurological function score,IL-10 level,p62 and mTOR protein levels increased(P<0.05).Compared with MCAO group,the number of autophagosomes,the protein levels of beclin-1,LC3 Ⅰ/Ⅱ and NLRP3 increased,while the protein levels of p62 and mTOR decreased in MCAO+RAPA group(P<0.05).Compared with MCAO+EA+Vector group,the protein levels of Beclin-1,LC3 Ⅰ/Ⅱ and NLRP3 in MCAO+EA+OE-mTOR group increased,while the protein levels of p62 and mTOR decreased(P<0.05).Conclusion:EA can improve cognitive function and inflammatory state in MCAO rats,and the mechanism may be related to the upregulation of mTOR/NLRP3 pathway and inhibition of autophagy.关键词
电针灸/脑卒中后认知障碍/自噬/雷帕霉素/NOD样受体蛋白3Key words
Electroacupuncture/Cognitive impairment after stroke/Autophagy/Rapamycin/Nod like receptor protein 3引用本文复制引用
袁红丽,牛相来,肖亚平,李秀娟,汪秀梅,周钰..电针灸通过mTOR/NLRP3介导的自噬改善卒中后认知功能障碍的研究[J].河北医学,2025,31(12):1998-2004,7.基金项目
国家自然科学基金,(编号:81960898) （编号:81960898）
新疆维吾尔自治区自然科学基金资助项目,(编号:2022D01C494) （编号:2022D01C494）
