湖南中医药大学学报2025，Vol.45Issue(12)：2307-2315,9.DOI:10.3969/j.issn.1674-070X.2025.12.009
基于HPLC指纹图谱和一测多评法的化瘀止痛贴膏质量评价
Quality evaluation of Huayu Zhitong Plaster based on HPLC fingerprint and QAMS method
摘要
Abstract
Objective To establish the HPLC fingerprint of Huayu Zhitong Plaster and to determine the content of its ten components using the quantitative analysis of multi-components by single-marker(QAMS)method,so as to provide a reference for quality assessment.Methods The HPLC fingerprint was developed using an Agilent HC-C18 column with a mobile phase of acetonitrile-1%formic acid 0.1%triethylamine under gradient elution conditions.The column temperature was maintained at 30℃and the flow rate was set at 1 mL·min-1.Similarity evaluation was performed on the fingerprint,and chemometric methods were applied for chemical pattern recognition.The content of the components were calculated using relative correction factors(RCFs).The results obtained by the QAMS method were compared with those determined by the external standard method,and additional RCFs were calculated accordingly for content determination.Results A total of ten common peaks were identified in the fingerprints of the 14 batches,with similarity evaluations exceeding 0.9.Using baicalin as the internal reference substance,the correction factors for catechin,hydroxysafflor yellow A,phellodendrine,gentiopicroside,magnoflorine,jateorrhizine,berberine,paeonol,and physcion were determined as 0.922 9,1.148 7,1.171 6,1.532 7,1.113 5,0.316 8,0.200 6,0.389 4,and 0.594 4,respectively.There was no significant difference between the results calculated by the QAMS method and those calculated by the external standard method.Conclusion The established HPLC fingerprint combined with the QAMS method demonstrates accuracy and reliability,thereby providing a scientific foundation for the quality control and comprehensive evaluation of Huayu Zhitong Plaster.关键词
化瘀止痛贴膏/质量评价/指纹图谱/一测多评/相似度评价Key words
Huayu Zhitong Plaster/quality evaluation/HPLC fingerprint/quantitative analysis of multi-components by single-marker/similarity evaluation分类
医药卫生引用本文复制引用
刘晓慧,彭江丽,奉艳婷,邓美玲,钱敏,王适,彭求贤..基于HPLC指纹图谱和一测多评法的化瘀止痛贴膏质量评价[J].湖南中医药大学学报,2025,45(12):2307-2315,9.基金项目
湖南省中医药管理局科研项目(B2023052) （B2023052）
湖南省自然科学基金-科药联合项目(2022JJ80087,2024JJ8175). （2022JJ80087,2024JJ8175）
