湖南中医药大学学报2025，Vol.45Issue(12)：2325-2334,10.DOI:10.3969/j.issn.1674-070X.2025.12.011
基于PGC-1α/NRF1信号通路探究点按脾俞穴对慢性疲劳综合征大鼠认知障碍的影响
Effects of acupressure at"Pishu(BL20)"on cognitive impairment in rats with chronic fatigue syndrome based on the PGC-1α/NRF1 signaling pathway
摘要
Abstract
Objective To explore the mechanism of action of acupressure at"Pishu(BL20)"on cognitive impairment in rats with chronic fatigue syndrome(CFS)based on the peroxisome proliferator-activated receptor γ coactivator-1α(PGC-1α)/nuclear respiratory factor 1(NRF1)pathway.Methods Thirty-two rats were randomly divided into blank group(n=8)and modeling group(n=24).The CFS model was established in the modeling group using a multifactorial compound stimulation method for a modeling period of 21 days.After modeling,rats in the modeling group were randomly divided into model group,"Pishu(BL20)"acupressure group,and ginsenoside group,with eight rats in each group.The blank group and model group received the intraperitoneal injection of physiological saline at 1 mL/day.The"Pishu(BL20)"acupressure group received bilateral acupressure at"Pishu(BL20)"followed by additional intraperitoneal injection of physiological saline at 1 mL/day.The ginsenoside group received intraperitoneal injection of ginsenoside solution at 1 mL/day.All interventions were administered once daily for 14 consecutive days.Assessments were conducted before and after modeling,and after intervention,including rats'body weight measurement,semi-quantitative scores of general condition,exhaustive swimming test and Morris water maze experiment.After the intervention,the adenosine triphosphate(ATP)content in the hippocampal tissue of rats was measured by colorimetry,the malondialdehyde(MDA)content in the hippocampal tissue was determined by thiobarbituric acid(TBA)colorimetry method,the superoxide dismutase(SOD)activity in the hippocampal tissue was tested by water-soluble tetrazolium salt 1(WST-1)method,and the mRNA expressions of PGC-1α and NRF1 in the hippocampal tissue were measured by real-time fluorescence quantitative polymerase chain reaction(PCR).The protein expression levels of PGC-1α and NRF1 in the hippocampal tissue were determineded by Western blot.Results(1)Before modeling,there were no statistically significant differences in body weight,semi-quantitative scores of general condition,exhaustive swimming time,and related indicators of the Morris water maze experiment among different groups of rats(P>0.05).After modeling,compared with the blank group,rats in the model group,"Pishu(BL20)"acupressure group,and ginsenoside group all showed decreased body weight(P<0.01),increased semi-quantitative scores of general condition(P<0.01),shortened exhaustive swimming time(P<0.05 or P<0.01),prolonged escape latency in the Morris water maze experiment(P<0.05),decreased total distance traveled(P<0.05 or P<0.01)and number of platform crossings(P<0.01).There were no statistically significant differences in the above indicators among the model group,"Pishu(BL20)"acupressure group,and ginsenoside group(P>0.05).After intervention,compared with the blank group,rats in the model group exhibited decreased body weight(P<0.01),increased semi-quantitative scores of general condition(P<0.01),shortened exhaustive swimming time(P<0.05),prolonged escape latency in the Morris water maze experiment(P<0.05),decreased total distance traveled(P<0.01)and number of platform crossings(P<0.01).Compared with the model group,rats in the"Pishu(BL20)"acupressure group and ginsenoside group both showed increased body weight(P<0.01),decreased semi-quantitative scores of general condition(P<0.01),and shortened exhaustive swimming time(P<0.01),shortened escape latency in the Morris water maze experiment(P<0.01),increased total distance traveled(P<0.01),and number of platform crossings(P<0.05 or P<0.01).There were no statistically significant differences in the above indicators between the"Pishu(BL20)"acupressure group and ginsenoside group(P>0.05).(2)Compared with the blank group,rats in the model group showed decreased ATP content and SOD activity in the hippocampal tissue(P<0.05),increased MDA content(P<0.05),decreased protein expression levels of PGC-1α and NRF1(P<0.01)and mRNA expression levels of PGC-1α and NRF1(P<0.01).Compared with the model group,rats in the"Pishu(BL20)"acupressure group and ginsenoside group both showed increased ATP content and SOD activity in the hippocampal tissue(P<0.05),and decreased MDA content(P<0.05),increased protein expression levels of PGC-1α and NRF1(P<0.05 or P<0.01)and mRNA expression levels of PGC-1α and NRF1(P<0.01).Conclusion Acupressure at"Pishu(BL20)"can effectively ameliorate the fatigue state and cognitive impairment in rats with CFS.It increases ATP content and SOD activity while reducing MDA content in the hippocampal tissue.The mechanism of action may be related to the activation of the PGC-1α/NRF1 signaling pathway and the improvement of mitochondrial energy metabolism.关键词
慢性疲劳综合征/点按法/脾俞穴/过氧化物酶体增殖受体γ辅激活因子α/核呼吸因子1信号通路/线粒体能量代谢/认知障碍Key words
chronic fatigue syndrome/acupressure/"Pishu(BL20)"/PGC-1α/NRF1 signaling pathway/mitochondrial energy metabolism/cognitive impairment分类
医药卫生引用本文复制引用
谭若彤,谢文彬,杨尚林,谢玮琪,唐雨兰,李铁浪..基于PGC-1α/NRF1信号通路探究点按脾俞穴对慢性疲劳综合征大鼠认知障碍的影响[J].湖南中医药大学学报,2025,45(12):2325-2334,10.基金项目
湖南省自然科学基金面上项目(2023JJ30454) （2023JJ30454）
湖南中医药大学大学生创新创业训练项目(S202410541038). （S202410541038）
