中医药导报2025，Vol.31Issue(12)：1-7,7.DOI:10.13862/j.cn43-1446/r.2025.12.001
基于Wnt/β-catenin通路探讨三七复方对D-半乳糖致衰老大鼠肾脏的抗氧化作用及机制
Exploring the Antioxidant Effects and Mechanisms of Panaxnotoginseng Formula on D-Galactose-Induced Aging Rat Kidneys via the Wnt/β-Catenin Pathway
摘要
Abstract
Objective:To evaluate the antioxidant and protective effects of Panaxnotoginseng formula(PNF)on the kidneys and to explore its mechanism in delaying aging through antioxidant effects via the Wnt/β-catenin pathway.Methods:Totally 72 male Sprague-Dawley(SD)rats were randomly divided into control group,model group,vitamin E group,PNF low dose group,PNF medium dose group,and PNF high dose group,12 rats in each group,based on body weight.Except for the control group,all other groups received daily intraperi-toneal injections of D-galactose for four weeks to establish an aging model.During modeling,the control and model groups were given distilled water via oral gavage daily.The vitamin E group received vitamin E solu-tion.The PNF low dose group,PNF medium dose group,and PNF high dose group received low dose,medium dose and high dose of PNF solution respectively for four weeks.The mental state and coat color of the rats were observed daily,and body weight was measured weekly.After treatment,kidney indices,renal function(BUN,Cr,UA),oxidative stress markers(MDA and Q18-OHdG),antioxidant indicators(T-SOD,GSH-Px,CAT,T-AOC and GSH/GSSG),renal morphology(HE staining),and protein expression levels of Wnt/β-catenin pathway components(Wnt4,GSK-3β,p-GSK-3β,β-catenin,c-Myc)were measured.Results:After D-galactose modeling,compared with the control group,the model group showed a decreased kidney index,impaired renal function,significantly increased levels of peroxidation products(MDA and 8-OHdG),and significantly decreased antioxidant indicators(T-SOD activity,GSH-Px activity,CAT activity,T-AOC,and GSH/GSSG ratio).The protein expression of Wnt4,p-GSK-3β,β-catenin,and c-Myc in renal tissue increased,while GSK-3β expression decreased and the p-GSK-3β/GSK-3β ratio increased.Compared with the model group,PNF group significantly improved renal function,alleviated oxidative damage,enhanced systemic antioxidant capacity,reduced the protein expression of Wnt4,p-GSK-3β,β-catenin,and c-Myc,and decreased the p-GSK-3β/GSK-3β ratio.Conclusion:Panaxnotoginseng formula can mitigate oxidative stress,reduce oxidative damage and renal injury,thereby exerting an anti-aging effect in D-galactose-induced aging model rats.The mechanism may involve inhibiting the Wnt/β-catenin pathway,promoting the degradation of β-catenin,reducing its translocation into the nucleus and binding to TCF/LEF,and suppressing the expression of the TCF/LEF-regulated c-Myc gene.This leads to reduced ROS production,decreased systemic oxidative damage,and ultimately contributes to its antioxidant and anti-aging effects.关键词
衰老/抗氧化/三七复方/Wnt/β-catenin通路/氧化应激/大鼠Key words
aging/antioxidant/Panaxnotoginseng formula/Wnt/β-catenin pathway/oxidative stress/rat分类
医药卫生引用本文复制引用
王祯,李亚金,沈奕玮,王倍翔,董媛媛,金振辉,王玉英,王景霞..基于Wnt/β-catenin通路探讨三七复方对D-半乳糖致衰老大鼠肾脏的抗氧化作用及机制[J].中医药导报,2025,31(12):1-7,7.基金项目
国家重点研发计划中医药现代化研究重点专项(2018YFC1706803) （2018YFC1706803）
北京中医药薪火传承"新3+3"工程建设单位王玉英三名传承工作室(2023-SZ-F-22) （2023-SZ-F-22）
