中医药导报2025，Vol.31Issue(12)：8-13,74,7.DOI:10.13862/j.cn43-1446/r.2025.12.002
基于Wnt/β-catenin信号通路探讨加味肺复方对肺腺癌A549细胞荷瘤裸鼠的影响
Exploring the Effects of Jiawei Feifu Formula(加味肺复方)on Nude Mice Bearing Lung Adenocarcinoma A549 Cells Based on the Wnt/β-catenin Signaling Pathway
摘要
Abstract
Objective:To investigate the effects of Jiawei Feifu formula on tumor growth and the Wnt/β-catenin signaling pathway in nude mice bearing lung adenocarcinoma A549 cells.Methods:Totally 65 BALB/c-nu nude mice were used in the study,and 10 mice were randomly selected as the blank control group.The re maining 55 mice were inoculated with the human lung adenocarcinoma A549 cells subcutaneously in the right axillary fossa to establish the tumor-bearing mouse model.The 50 BALB/c-nu mice were divided into model group(distilled water),Jiawei Feifu formula low dose group,Jiawei Feifu formula medium dose group,Jiawei Feifu formula high dose group,and positive control group(cisplatin),10 mice in each.The tumor volume and weight were assessed.Western blotting(WB)was employed to detect the expression levels of β-catenin nuclear protein.Quantitative real-time PCR(qPCR)was used to detect the expression levels of c-Myc mRNA,c-jun mRNA,and CyclinD1 mRNA.Results:The Jiawei Feifu formula medium dose group,Jiawei Feifu formula high dose group,and positive control group showed lower tumor volume and tumor weight than model group(P＜0.01 or P＜0.05).No statistically significant difference was observed in tumor volume or tumor mass between the Jiawei Feifu formula low dose group and model group in nude mice(P＞0.05).The Jiawei Feifu formula high dose group,and positive control group showed lower relative expression levels of c-Myc mRNA,c-jun mRNA and Cyclin D1 mRNA than model group(P＜0.01 or P＜0.05).Compared with the model group,there were no statistically significant differences in the relative expression levels of c-Myc mRNA,c-jun mRNA and Cyclin D1 mRNA in the Jiawei Feifu formula low dose group,and Jiawei Feifu formula medium dose group(P＞0.05).The Jiawei Feifu formula low dose group,Jiawei Feifu formula medium dose group,Jiawei Feifu formula high dose group,and positive control group showed lower relative expression levels of β-catenin than model group(P＜0.01 or P＜0.05).Conclusion:Jiawei Feifu formula can inhibit tumor growth in mice bearing lung adenocarcinoma A549 cells,and the tumor-inhibiting effect is concentration-dependent.Its mechanism may be related to the regulation of the Wnt/β-catenin signaling pathway.关键词
肺腺癌/加味肺复方/A549细胞/Wnt/β-catenin信号通路/裸鼠Key words
lung adenocarcinoma/Jiawei Feifu formula/A549 cells/Wnt/β-catenin/signaling pathway/nude mice分类
医药卫生引用本文复制引用
胡玉田,潘博,傅剑锋,潘敏求..基于Wnt/β-catenin信号通路探讨加味肺复方对肺腺癌A549细胞荷瘤裸鼠的影响[J].中医药导报,2025,31(12):8-13,74,7.基金项目
国家中医药管理局第四届国医大师潘敏求传承工作室(国中医药办人教函[2022]245号) （国中医药办人教函[2022]245号）
