湖南畜牧兽医Issue(6)：27-30,4.DOI:10.3969/j.issn.1006-4907.2025.06.008
一起猪轮状病毒感染的实验室诊断及VP4、VP7基因序列分析
Laboratory Diagnosis of a Case of Porcine Rotavirus and Sequence Analysis of VP4 and VP7 Genes
摘要
Abstract
To investigate the cause of diarrhea in piglets at a large-scale pig farm in the Aksu region of Xinjiang,intestinal contents from deceased piglets were collected and subjected to differential detection of Porcine pidemic iarrhea irus(PEDV),Transmissible astroenteritis virus(TGEV),and Porcine otavirus(PoRV)using quantitative real-time RT-PCR.The results showed that the samples tested positive for Porcine rotavirus,indicating that the pathogen responsible for diarrhea in this farm was Porcine rotavirus.Specific primers targeting the VP4 and VP7 gene sequences of the PoRV strain were designed and synthesized.The corresponding gene fragments were amplified via RT-PCR,and the amplification products were sequenced and analyzed for homology.The corresponding gene fragments were amplified via RT-PCR,and the amplification products were sequenced and analyzed for homology.The results revealed that the VP4 gene sequence of this strain shared 82.0%to 92.3%nucleotide homology with P[6]reference strains,while the VP7 gene sequence exhibited 93.1%nucleotide homology with G4 reference strains.Based on these findings,the pathogen causing this outbreak was identified as a G4 P[6]genotype porcine rotavirus.This study provides important insights for the prevention and control of the disease.关键词
规模化猪场/仔猪腹泻/轮状病毒/VP4/VP7Key words
Large-scale pig farm/Piglet diarrhea/Rotavirus/VP4/VP7分类
农业科技引用本文复制引用
周锋涛,刘洋,张振,刘淑华,李莲瑞..一起猪轮状病毒感染的实验室诊断及VP4、VP7基因序列分析[J].湖南畜牧兽医,2025,(6):27-30,4.基金项目
新疆生产建设兵团科技特派员创新创业示范项目(2018CB037) （2018CB037）
