生物灾害科学2025,Vol.48Issue(4):541-547,7.DOI:10.3969/j.issn.2095-3704.2025.04.67
油茶炭疽病病原鉴定研究
Research on pathogen identification of Camellia oleifera anthracnose
摘要
Abstract
[Objective]This study aimed to identify the main pathogens of Camellia oleifera anthracnose,so as to provide a theoretical basis for the scientific prevention and control of this disease.[Method]Pathogens were isolated and identified from diseased leaf samples collected from the Ca.oleifera planting base of Jiangxi Agricultural University in Nanchang City,Jiangxi Province.Morphological and molecular biological methods were used,combining the colony morphology of pathogens,conidium morphology,multi-phylogenetic tree analysis,and pathogenicity tests to clarify the species of pathogens.[Result]Based on the gene sequence analysis of ACT(actin),GAPDH(glyceraldehyde-3-phosphate dehydrogenase),ITS(internal transcribed spacer)and morphological identification,the pathogens causing Ca.oleifera anthracnose were determined to be Colletotrichum fructicola and Colletotrichum siamense.[Conclusion]This study confirmed that the pathogens responsible for anthracnose in the Ca.oleifera planting area of Jiangxi Agricultural University in Nanchang City,Jiangxi Province,were C.fructicola and C.siamense.The results provide a theoretical foundation for the subsequent comprehensive prevention and control of this disease.关键词
油茶/炭疽病/分离/病原鉴定Key words
Camellia oleifera/anthracnose/isolation/pathogen identification分类
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李宜美,万良鹏,高文娜,刘心怡,陈芸嫣,苏楊茵,崔汝强,况卫刚..油茶炭疽病病原鉴定研究[J].生物灾害科学,2025,48(4):541-547,7.基金项目
江西省教育厅青年项目(GJJ170294)和江西农业大学大学生创新创业项目(202410410017) (GJJ170294)