华南农业大学学报2026，Vol.47Issue(1)：1-10,10.DOI:10.7671/j.issn.1001-411X.202507015
3种益生菌对牛蛙生长性能、血液生化指标、免疫抗氧化能力及肠道健康的影响
Effects of three probiotics on growth performance,blood biochemical indexes,immune and antioxidant capacity and intestinal health of Aquarana catesbeiana
摘要
Abstract
[Objective]This study aims to investigate the effects of dietary probiotics on growth performance,blood biochemical indices,immune and antioxidant capacity,and intestinal health in Aquarana catesbeiana.[Method]The control group was fed the basal diet.Experimental diets were formulated by supplementing with Clostridium butyricum(2.92×107 cfu/g),Bacillus coagulans(0.91×107 cfu/g),or Saccharomyces cerevisiae(the mass fraction was 0.2%).A total of 432 A.catesbeiana with an initial body weight of(38.94±0.04)g were randomly assigned to four groups with three replicates of 36 frogs each.The feeding trial lasted for 56 days.[Result]1)Survival rate,feed conversion ratio,and protein deposition rate showed no significant differences among groups.The C.butyricum group achieved significantly higher final weight per frog,weight gain rate,and specific growth rate compared to other three groups.2)Trypsin(TRY)activity of liver or intestine in all test groups was higher than that in the control,with the C.butyricum and S.cerevisiae groups showing significantly higher activities and the C.butyricum group being the highest.3)The C.butyricum group showed significantly higher total cholesterol(T-CHO)concentration and significantly lower aspartate aminotransferase(AST)activity.The S.cerevisiae group had significantly elevated blood glucose(GLU)concentration.The control group exhibited significantly higher triglyceride(TG)concentration and alanine aminotransferase(ALT)activity than three test groups.4)Compared to the control group,A.catesbeiana in the test groups had significantly higher levels of liver antioxidant indices including alkaline phosphatas(AKP),total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px)activities and total antioxidant capacity(T-AOC),intestinal lysozyme(LZM)activity,and serum immune indices(LZM and AKP activities,T-AOC).The C.butyricum group demonstrated the best performance in liver catalase(CAT)activity,intestinal AKP activity,and most immune-antioxidant parameters,its liver malondialdehyde(MDA)content and serum T-AOC did not differ significantly from other groups,whereas liver MDA content was significantly elevated in the control group.Additionally,liver LZM activity significantly increased in the S.cerevisiae group compared to the control group.[Conclusion]Dietary supplementation with C.butyricum,B.coagulans,or S.cerevisiae improves digestive enzyme activity,immune function and antioxidant capacity,and intestinal villus growth in A.catesbeiana,with C.butyricum showing the best overall efficacy.关键词
牛蛙/丁酸梭菌/凝结芽孢杆菌/酿酒酵母/生长性能/生化指标/免疫抗氧化Key words
Aquarana catesbeiana/Clostridium butyricum/Bacillus coagulans/Saccharomyces cerevisiae/Growth performance/Biochemistry indice/Immune anti-oxidation分类
农业科技引用本文复制引用
梁冠宇,熊攀,陈智兵,李琳娜,蔡云川,莫昌盛,王国霞..3种益生菌对牛蛙生长性能、血液生化指标、免疫抗氧化能力及肠道健康的影响[J].华南农业大学学报,2026,47(1):1-10,10.基金项目
广东省饲料产业技术体系(2024CXTD14) （2024CXTD14）
2023年广东省企事业单位委托项目(横015) （横015）
