华南农业大学学报2026，Vol.47Issue(1)：52-63,12.DOI:10.7671/j.issn.1001-411X.202506004
越南蔷薇菌YJ21菌株鉴定及生防活性测定
Identification and biocontrol assay of Rossellomorea vietnamensis strain YJ21
摘要
Abstract
[Objective]To screen bacteria from mangrove soil that can inhibit the growth and development of Sporisorium scitamineum,Magnaporthe oryzae and Phytophthora capsici while promoting plant growth,thus providing biocontrol resources for managing crop diseases caused by these pathogens.[Method]Bacteria were isolated from mangrove soil using the dilution spread plate method.The species of bacteria was identified through 16S rRNA sequencing and whole genome sequencing.The antagonistic effects of the bacteria against phytopathogens were evaluated via plate confrontation assay,spore germination test,in vitro leaf inoculation test,and pot inoculation experiment.Salkowski colorimetry was used to determine the indole-3-acetic acid(IAA)production capacity of isolated bacterial strain,and the promotive effect of strain on rice seed germination was evaluated via the seed soaking assay.[Result]Strain YJ21 was isolated from the mangrove soil.It was identified as Rossellomorea vietnamensis through genomic evolutionary analysis.The inhibition rates of the YJ21 strain on the mycelial growth of M.oryzae and P.capsici were 34.62%and 39.87%,respectively.Its fermentation supernatant inhibited the teliospore germination in S.scitamineum teliospores,the inhibition rate of conidial germination in M.oryzae reached 90.48%,and the supernatant also reduced the pathogenicity of M.oryzae on rice.Furthermore,the YJ21 strain was capable of producing IAA and protease,and it significantly promoted the growth of root and shoot after rice seed germination.[Conclusion]R.vietnamensis YJ21,isolated from mangrove soil,has a potential application for the biocontrol of rice blast.关键词
越南蔷薇菌/红树林沉积物/稻瘟病/生物防治Key words
Rossellomorea vietnamensis/Mangrove sediments/Rice blast/Biocontrol分类
农业科技引用本文复制引用
陈楚滟,尹凯,张腾宇,胡志剑,陈圣文,梁志彬,邓懿祯..越南蔷薇菌YJ21菌株鉴定及生防活性测定[J].华南农业大学学报,2026,47(1):52-63,12.基金项目
国家自然科学基金区域创新发展联合基金(U23A20148) （U23A20148）
