农业科技与技术（英文）2025，Vol.26Issue(3)：6-14,9.DOI:10.16175/j.cnki.1009-4229.2025.03.002
甘蓝型油菜BnOPR1基因克隆及表达载体构建
Cloning and Expression Vector Construction of BnOPR1 from Brassica napus
摘要
Abstract
The 12-oxophytodienoate reductase(OPR),a flavin mononucleotide-dependent oxidoreductase,regulates plant responses to stress conditions such as heavy metals,drought,saline-alkali,pests,and diseases by participating in the synthesis of plant hormones such as jasmonic acid(JA).In this study,homologous cloning was employed to obtain the Brassica napus'Zhongshuang 11'OPR1 gene(BnOPR1).Bioinformatics analysis was conducted for the deduced protein BnOPR1,and a plant expression vector for BnOPR1 was constructed.The bioinformatics analysis revealed that BnOPR1 was 1 125 bp in length,encoding 375 amino acid residues.The deduced protein had a molecular weight of 41.604 kDa,an isoelectric point of 6.05,a molecular formula of C1853H2855N511O550S16,an aliphatic index of 35.47,a lipophilicity index of 74.87,and an instability index of 39.49.It had 49 phosphorylation sites and lacked transmembrane domains and signal peptides.The phylogenetic analysis indicated that BnOPR1 had a close relationship with the OPR1 protein from Brassica rapa since they shared the same clade,while it had a distant relationship with the OPR1 protein from Raphanus sativus.In this paper,the expression vector for BnOPR1 was successfully constructed by seamless cloning and named pBnOPR1.The findings laid a foundation for further studying the roles of BnOPR1 in the response to antimony stress.关键词
甘蓝型油菜/BnOPR1 基因/同源克隆/载体构建Key words
Brassica napus/BnOPR1 gene/Homologous cloning/Vector construction引用本文复制引用
程一苑,袁玉辉,王艳红,龚莎,凌荟,黄靖依,钟品田,刘显军..甘蓝型油菜BnOPR1基因克隆及表达载体构建[J].农业科技与技术（英文）,2025,26(3):6-14,9.基金项目
Supported by China National University Student Innovation&Entrepreneurship Development Program(S202410553003) （S202410553003）
Natural Science Foundation of Hunan Province(2025JJ70318,2023JJ50083). 国家大学生创新创业项目专项(S202410553003) （2025JJ70318,2023JJ50083）
湖南省自然科学基金项目(2025JJ70318,2023JJ50083) （2025JJ70318,2023JJ50083）
