农业科技与技术（英文）2025，Vol.26Issue(3)：24-30,7.DOI:10.16175/j.cnki.1009-4229.2025.03.004
芍药PlPR1抵御细极链格孢菌侵染的功能鉴定
Functional Analysis of PlPR1 in Defending Against the Infection of Alternaria tenuissima
摘要
Abstract
Leaf spot disease caused by Alternaria tenuissima seriously affects the yields of medicinal roots and cut flowers of Paeonia lactiflora.To mine the leaf spot resistance genes in P.lactiflora,we employed rapid amplification of cDNA ends(RACE)to clone PlPR1 from the leaves of P.lactiflora.The results of bioinformatics analysis showed that the coding region of PlPR1 was 522 bp in length,and the encoded protein contained a typical CAP domain.Overexpression of PlPR1 in tobacco significantly decreased the disease index,delayed the disease progression,and attenuated the hypersensitive response induced by pathogen invasion in the transgenic plants.In conclusion,PlPR1 enhanced the plant resistance to the pathogen A.tenuissima by regulating the immune responses of plants.The findings provided a candidate target gene and a theoretical basis for breeding P.lactiflora varieties with disease resistance.关键词
芍药/细极链格孢菌/PlPR1/功能鉴定Key words
Paeonia lactiflora/Alternaria tenuissima/PlPR1/Functional identification引用本文复制引用
朱永芳,姜玉蕾,郭先锋..芍药PlPR1抵御细极链格孢菌侵染的功能鉴定[J].农业科技与技术（英文）,2025,26(3):24-30,7.基金项目
Supported by the Natural Science Foundation of Shandong Province(ZR2021MC007) 山东省自然科学基金项目(ZR2021MC007) （ZR2021MC007）
