军事医学2025，Vol.49Issue(11)：801-811,11.DOI:10.7644/j.issn.1674-9960.2025.11.001
间隔微波辐射对阿尔兹海默病小鼠认知功能及病理结构的改善作用研究
Effects of intermittent microwave irradiation on cognitive function and pathological structure of AD mice
摘要
Abstract
Objective To investigate the effects of intermittent microwave irradiation on pathological structure and cognitive function of Alzheimer's disease(AD)mice.Methods Forty C57BL/6J mice and another forty 5×FAD mice were randomly divided into the sham irradiation(sham-WT),irradiation(MW-WT),AD sham irradiation(sham-AD),and AD irradiation(MW-AD)groups,with 20 mice in each.Mice underwent daily 1-hour microwave irradiation(900 MHz,40 Hz repetition rate)on an intermittent schedule:2 weeks on,2 weeks off,and 2 weeks on again.The cognitive function of mice was assessed via behavioral experiments conducted both immediately and six weeks after irradiation.The expressions of phosphorylated tau(p-Tau)and b amyloid protein(Aβ)plaques were detected via immunofluorescence.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of key enzymes involved in Aβ production and neuroinflammatory factors in the brain tissue.Results Compared with the sham-WT group,the novel object recognition index and the novel arm recognition index in the sham-AD group were significantly reduced(P<0.05),but those were significantly increased in the MW-AD group compared to the sham-AD group(P<0.05).In addition,the levels of p-Tau and Aβ proteins in the brain of the MW-AD group were significantly decreased(P<0.05)while the levels of β-secretase and γ-secretase in the cortex were significantly increased(P<0.05).The levels of CD68,tumor necrosis factor-α(TNF-α),and interleukin-10(IL-10)were also increased to varying degrees(P<0.05).Conclusion Intermittent microwave irradiation(900 MHz,40 Hz)can ameliorate cognitive deficits and attenuate Aβ and p-Tau pathology in AD mice rather than inhibit the key secretases involved in Aβ production or the occurrence of neuroinflammation.关键词
阿尔兹海默病/微波/认知功能/β-淀粉样蛋白沉积/tau蛋白磷酸化Key words
Alzheimer's disease/microwave/cognitive function/β amyloid protein deposition/phosphorylated tau protein分类
医药卫生引用本文复制引用
张铭钊,杨臻琪,邹勇,马丽贞,智维佳,牛佳佳,董霁,胡向军,王丽峰..间隔微波辐射对阿尔兹海默病小鼠认知功能及病理结构的改善作用研究[J].军事医学,2025,49(11):801-811,11.基金项目
国家自然科学基金面上项目(62171457) （62171457）
