军事医学2025，Vol.49Issue(11)：846-852,7.DOI:10.7644/j.issn.1674-9960.2025.11.006
基于侧流免疫层析技术的NSE和S100β联合检测方法的建立
Establishment of a combined detection method for NSE and S100β based on lateral flow immunochromatography technology
摘要
Abstract
Objective To establish a quantum dot immunochromatographic test strip for simultaneous detection of central nervous system-specific protein(S100 β)and neuron-specific enolase(NSE),thereby achieving combined detection of both biomarkers.Methods A double-antibody sandwich immunochromatographic assay was developed using S100β and NSE capture antibodies as the test line and species-specific secondary antibodies as the control line coated on the nitrocellulose membrane.Quantum dot bead-conjugated antibodies targeting S100β and NSE served as fluorescent probes.The preparation parameters of the strip were optimized,and its performance was systematically evaluated to develop a rapid diagnostic strip for mild traumatic brain injury(mTBI).The diagnostic efficacy of the strip was further validated using clinical samples.Results The total assay time for combined detection of S100β and NSE was 17 min,with a limit of detection(LOD)of 0.1 ng/mL for S100β and 2.67 ng/mL for NSE.The inter-batch reproducibility of the strips was excellent(coefficient of variation,CV<15%).In clinical validation using 24 mTBI samples,the combined detection strip demonstrated a specificity of 90%and a sensitivity of 92.86%,outperforming single-marker detection.These results prove that the simultaneous quantification of S100β and NSE enhances diagnostic efficacy for mTBI patients.Conclusion A combined detection method for the biomarkers S100β and NSE in mTBI,based on quantum dot immunochromatography,has been preliminarily established.This method demonstrates promising clinical application prospects in the auxiliary diagnosis of mTBI.关键词
轻度颅脑损伤/联合检测/神经元特异性烯醇化酶/中枢神经特异性蛋白/量子点免疫层析Key words
mild traumatic brain injury/combined detection/neuron-specific enolase/central nervous system specific protein β/quantum dot immunochromatography分类
医药卫生引用本文复制引用
孙若媛,陈昌国,邢微微,刘中成,徐东刚,付文亮,张超,夏文戎,赵永岐,高波,张军,刘爱军..基于侧流免疫层析技术的NSE和S100β联合检测方法的建立[J].军事医学,2025,49(11):846-852,7.基金项目
河北省自然科学基金项目(H2023201026,H2022201035) （H2023201026,H2022201035）
