口腔颌面外科杂志2025，Vol.35Issue(6)：448-455,8.DOI:10.12439/kqhm.1005-4979.2025.06.004
衰老对颌骨骨缺损再生影响的实验研究
Effects of aging on the regeneration of jawbone defects
摘要
Abstract
Objective:To investigate the effects of aging on the bone regeneration capacity of mouse jaw and the osteogenic differentiation ability of mouse embryo osteoblast precursor cells(MC3T3-E1 cells).Methods:A maxillary bone defect model was established using senescence-accelerated mice(SAMP8 mice).Micro-CT was used to analyze the bone microstructural parameters of new bone in the maxillary bone defect area of young(2-month-old)and aged(8-month-old)mice.In vitro,hydrogen peroxide(H2O2)was used to induce senescence in MC3T3-E1 cells.The expression of senescence-related genes was detected by real-time quantitative polymerase chain reaction(RT-qPCR)and Western blotting.Senescence-associated β-galactosidase(SA-β-Gal)staining was used to compare the positive area;flow cytometry was employed to analyze cell cycle distribution;alkaline phosphatase(ALP)staining and alizarin red S(ARS)staining were used to evaluate osteogenic differentiation ability;and Western blotting was applied to detect the expression levels of osteogenesis-related proteins.Results:On the 14th day after bone defect surgery,the amount of new bone in the aged group was lower than that in the young group.In vitro experiments showed that after H2O2 treatment,the expression levels of senescence-related genes and proteins in MC3T3-E1 cells were significantly increased,and the SA-β-Gal staining positive area was significantly enlarged.Cell cycle analysis revealed a significant decrease in the proportion of cells in the S and G2 phases.Meanwhile ALP and ARS staining indicated weakened osteogenic differentiation,and the expression levels of osteogenesis-related proteins,bone morphogenetic protein-2(BMP-2)and Runt-related transcription factor 2(Runx2)were also significantly decreased.Conclusion:Aging can inhibit bone regeneration in the mouse jaw,partly due to the decreased differentiation capacity of osteoblast precursor cells under aging conditions.关键词
骨缺损/衰老/骨再生/SAMP8小鼠Key words
bone defect/aging/bone regeneration/SAMP8 mice分类
医药卫生引用本文复制引用
徐亦凡,王佐林..衰老对颌骨骨缺损再生影响的实验研究[J].口腔颌面外科杂志,2025,35(6):448-455,8.基金项目
国家重点研发计划(2018YFE0202200) （2018YFE0202200）
中央高校基本业务费专项资金(22120180196) （22120180196）
国家自然科学基金(81670962) （81670962）
